LOCUS CK334651 563 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075H07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075H07 5', mRNA sequence. ACCESSION CK334651 VERSION CK334651.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 563) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3075 row: H column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..563 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075H07-5" /db_xref="taxon:10090" /clone="H3075H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 101 a 167 c 178 g 117 t ORIGIN 1 aggcccacat gcaggcccgg tttgtgatcc tgcgggtttt gctggaagct ggcgaaggac 61 tcgttaccgt cactcccacc acaggctctg atgggcgccc tgatgcccgg gtccgcctgg 121 accgcagcaa gatccggtca gtgggcagac ctgccttgga gcggttcctg cggagactcc 181 aggtgctgaa gtccacgggg gacgtggtcg cggggcgggc cctgtacgag ggctatgcag 241 cagtcactga cgcacctcca gagtgcttcc ttaccctaag ggatacggta ctgctgcgca 301 aggagtcccg gaagcttatt gttcaaccca acactcgcct tgaaggctca gaagtacaac 361 ttgtggagta tgaggcctca gccgctggtc tcatccgctc cttctgtgag cggtttcccg 421 aggatgggcc cgagttggag gaggttctta tccagctggc tgcagcagat gcccgattct 481 ggaggaacca ggcccaggag gccccacctg gccaggcttg agaagatctg tgtggtctct 541 cccctctttg tcaaaccagg gct //