LOCUS CK334597 523 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061E05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061E05 3', mRNA sequence. ACCESSION CK334597 VERSION CK334597.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 523) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3061 row: E column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..523 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061E05-3" /db_xref="taxon:10090" /clone="H3061E05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 143 a 108 c 113 g 159 t ORIGIN 1 aaaaattttt tattcactaa tatgtggagg tgtgactttg gatccagcca gtcagtgata 61 taaataaggt tgagcaaaaa gattaaagct agagagtgta ggtgtgtgca tttgtatatt 121 tgtatgtgtg cataaggctt cttggagcag tgccacaaac gtggacacca atgaaaaata 181 tcactgccgt cctttgaaat ttccatgaag agtacaatgc gggcaattat atgaggatgc 241 tctaaatggc tctttccatc tcctgcactc acatgcccgc caaacatgaa atgttcgcct 301 tctcccttcc aaagtgatgg ttgttgaact tatttttagt gtcatttgat aagcctttgt 361 gctcgcagga gacatcccac tgacccggcc actggtcatt gtctataaca gttcacatca 421 aagcaggcgc gctttgtcag gtttccagtc gaatatccat ttcgcatctg aagtacagta 481 tcgaaagtga ctagattatc tgagctattt ccttcggcag ctg //