LOCUS CK334505 561 bp mRNA linear EST 12-MAY-2010 DEFINITION H3042D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3042D04 5', mRNA sequence. ACCESSION CK334505 VERSION CK334505.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 561) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3042 row: D column: 04 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..561 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3042D04-5" /db_xref="taxon:10090" /clone="H3042D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 122 a 153 c 175 g 111 t ORIGIN 1 tgctccctga aaccgctttc tggcgctgct ggtggggtga gcgctggttg ccgagggttt 61 cggtcccaac tgctgtcagt agtgatccag ccatccccag tcctgcgccg tcggggctcc 121 ccgcctccgc tgaagaacgg gaccatggaa gacaaaccac tggtagtatc taaacagaag 181 acagaagtgg tgtgcggtgt gcccacccag gtggtctgca cggccttcag cagccacatc 241 ctagttgtgg tgacccagtt cgggaagatg ggtacgctag tgtccttgga gcccagcaat 301 gtggccaatg acatcagcaa gccagtgctc accacgagag tcctcctcgg gcaggacgag 361 cctctcatcc atgtctttgc aaagaacctg gtagcatttg tgtcgcaaga agcaggaaac 421 agagccgtcc tgctggccat ggctgtgaaa gacaagagca tggagaggct caaggcattg 481 aaggaagtga tccggctgtg ccaggtgtgg tgacctgccg ttagcagtgc cagacacaga 541 cgtatggacg ctcggtgagc g //