LOCUS CK334472 624 bp mRNA linear EST 12-MAY-2010
DEFINITION H3034G09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3034G09 3', mRNA sequence.
ACCESSION CK334472
VERSION CK334472.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 624)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3034 row: G column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..624
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3034G09-3"
/db_xref="taxon:10090"
/clone="H3034G09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 165 a 124 c 104 g 231 t
ORIGIN
1 gtgttctaag agatatttgt caatgaaagt tataaaaata ctacttcatt ttgtcttctg
61 gaagctttct actgttaacc atgaacacct gcaccccagt aaaacggaaa ctgatttgca
121 atctggtgag aggtagttca agcttcctgg ggttttttct ttgtgtgtcc aaattatttt
181 agctaagcta caggtttacc ttttgttaca agttgagttt ttccccactg gtcagtatag
241 ctaatattcg cccaacagta gtgtcttctc tattgcagtt ttacactagg tgccacctcc
301 agactctgcc ttctactctg ttcctcatta ctgctttaga tatttttggt cctttgtgga
361 aagctgccat ttatactttt aaaacattgt caattcccaa atttaaaact gtactaaagc
421 taaaaaggcc catttgatca ctaatgtcac aaagctatct ttacttcatg agtatcatat
481 agtccttact tgggctgaca ttaacactca ttagttctct atattggtat cttgaacacc
541 tactaaattt gcgtgggatc tgataaagta gttattgtta ttggttgtgg tcttaaaatg
601 tcctccttag gctcatattt gaac
//