LOCUS CK334464 576 bp mRNA linear EST 12-MAY-2010 DEFINITION H3032F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3032F06 3', mRNA sequence. ACCESSION CK334464 VERSION CK334464.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 576) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3032 row: F column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..576 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3032F06-3" /db_xref="taxon:10090" /clone="H3032F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 145 a 154 c 146 g 131 t ORIGIN 1 gcagtgaaat aaatgctttt tatttgtcag aagacagatt ttcaaatgtc tttatcattt 61 tgccaacaac tgacagatgc tctcttggga acaattaacc attgttcaca ggcaagagca 121 ggaaaggggg tttagtggta cttgtgagcc agggcagcgg ccactccagc caccaccttc 181 tggaaggcag cctgtgcagc gggggtgaaa tccttgccca ggtggtggcc cagcacaatc 241 acgatcatat tgcccaggag cctgaagttc tcaggatcca catgcagctt gtcacagtgg 301 agctcactga ggctggcaaa ggtgcccttg aggctgtcca agtgattcag gccatcgtta 361 aaggcagtta tcactttctt gccatgggcc ttcactttgg cattacccat gatagcagag 421 gcagaggata ggtctccaaa gctatcaaag taccgctggg tccaagggta gacaaccagc 481 agcctgccca gggcctcacc accaacttca tcggcgttca cctttcccca caggccagag 541 acagcagcct tctcagcatc agtcaggtgc accatg //