LOCUS CK334424 582 bp mRNA linear EST 12-MAY-2010 DEFINITION H3025H07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3025H07 5', mRNA sequence. ACCESSION CK334424 VERSION CK334424.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 582) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3025 row: H column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..582 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3025H07-5" /db_xref="taxon:10090" /clone="H3025H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 146 c 158 g 101 t ORIGIN 1 gccatggcac ctccgggagg aaagatcaat cgtccccgga cggagctgaa gaagaagttg 61 ttcaagcgcc gcagggtatt aagcagggat cggcgacgga aacgccaggt ggtcggggct 121 gtgatagacg aagggctgac tacgaagcac cacctcaaga agcgggcgtc cagtgctcgt 181 gccaacatca cgctgtctgg gaagaagcgc aggaaactct tgcagcagat ccgacttgcc 241 cagaaagaaa aagcagccat ggaagtggaa gccccctcca agtcaaccag gactagtcag 301 ccacagccca agcaacaaaa gaagataaaa gctccccagg acgtagctat ggaggatctt 361 gaagataaga gctaaaatcc ctgcctcttc taccagaaga tggtcaacag cactgagaaa 421 gatttggagg aactttggaa aaaatattag catatttcgc tttcctagac acctcttcta 481 cagcggaccc tgacctcccc tggaggtgta tattgggacc gaagagggca cagagaaaga 541 ctggagggcc acctctagca gtcaactcct tttgtaataa ag //