LOCUS CK334394 592 bp mRNA linear EST 12-MAY-2010 DEFINITION H3021F07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3021F07 5', mRNA sequence. ACCESSION CK334394 VERSION CK334394.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3021 row: F column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3021F07-5" /db_xref="taxon:10090" /clone="H3021F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 116 a 159 c 184 g 133 t ORIGIN 1 gcggacgctg ctggggctgg ggctgctggt tgcgggctcg cgcctgccac gggtcatcag 61 ccagcagagt gtgtgtcgtg caaggcccat ctggtgggga acacagcgcc ggggctcgga 121 gaccatggcg ggcgctgcgg tgaagtactt aagtcaggag gaggctcagg ccgtggacca 181 agagcttttt aacgagtatc agttcagcgt ggatcaactc atggagctgg ccgggttgag 241 ctgtgccacg gctattgcca aggcttatcc ccccacgtct atgtccaaga gtcccccgac 301 tgtcttggtc atctgtggcc ccggaaataa cggaggggat gggctggtct gtgcgcgaca 361 cctcaaactt tttggttacc agccaactat ctattacccc aaaagaccta acaagcccct 421 cttcactggg ctagtgactc agtgtcagaa aatggacatt cctttccttg gtgaaatgcc 481 cccagagccc atgatggtgg acgagctgta tgagctggtg gtggacgcca tcttcggctt 541 cagtttcaag ggtgacgttc gggagccatt ccacagcatc ctgagtgtct tg //