LOCUS CK334375 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020D07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020D07 5', mRNA sequence. ACCESSION CK334375 VERSION CK334375.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3020 row: D column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020D07-5" /db_xref="taxon:10090" /clone="H3020D07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 202 a 151 c 164 g 144 t ORIGIN 1 aacgcaggtg ttgtgaaaac caccgctaat tcaaagcaaa aatgggaaag gaaaagactc 61 acatcaacat cgtcgtaatc ggacacgtag attccggcaa gtccaccaca accggccacc 121 tgatctacaa atgtggtgga atcgacaagc gaaccatcga aaagtttgag aaggaggctg 181 ctgagatggg aaagggctcc ttcaagtacg cctgggtctt agacaaactg aaagctgagc 241 gtgagcgtgg tatcactatt gacatctccc tgtggaaatt cgagaccagc aaatactatg 301 tgaccatcat tgatgcccca ggacacagag acttcatcaa aaacatgatt acaggcacat 361 cccaggctga ctgtgctgtc ctgattgttg ctgctggtgt tggtgaattt gaagctggta 421 tctccaagaa cgggcagacc cgcgagcatg ctcttctggc ttacaccctg ggtgtgaaac 481 agctgattgt tggtgtcaac aaaatggatt ccaccgagcc accatacagt cagaagagat 541 acgaggaaat cgttaaggaa gtcagcacct acattaagaa aattggctac aaccctgaca 601 cagtagcatt tgtgccaatt tctggttgga atggtgacaa catgctggag ccaagtgcta 661 a //