LOCUS CK334364 627 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019E08 5', mRNA sequence. ACCESSION CK334364 VERSION CK334364.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 627) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3019 row: E column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..627 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019E08-5" /db_xref="taxon:10090" /clone="H3019E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 148 a 176 c 171 g 132 t ORIGIN 1 gcagtgccag cctcgtcccg tagacaaaat ggtgaaggtc ggtgtgaacg gatttggccg 61 tattgggcgc ctggtcacca gggctgccat ttgcagtggc aaagtggaga ttgttgccat 121 caacgacccc ttcattgacc tcaactacat ggtctacatg ttccagtatg actccactca 181 cggcaaattc aacggcacag tcaaggccga gaatgggaag cttgtcatca acgggaagcc 241 catcaccatc ttccaggagc gagaccccac taacatcaaa tggggtgagg ccggtgctga 301 gtatgtcgtg gagtctactg gtgtcttcac caccatggag aaggccgggg cccacttgaa 361 gggtggagcc aaaagggtca tcatctccgc cccttctgcc gatgccccca tgtttgtgat 421 gggtgtgaac cacgagaaat atgacaactc actcaagatt gtcagcaatg catcctgcac 481 caccaactgc ttagcccccc tggccaaggt catccatgac aactttggca ttgtggaagg 541 gctcatgacc acagtccatg ccatcactgc cacccagaag actgtggatg gcccctctgg 601 aaagctgtgg cgtgatggcc gtggggc //