LOCUS CK334322 589 bp mRNA linear EST 12-MAY-2010 DEFINITION H3016E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3016E08 5', mRNA sequence. ACCESSION CK334322 VERSION CK334322.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3016 row: E column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3016E08-5" /db_xref="taxon:10090" /clone="H3016E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 188 c 132 g 115 t ORIGIN 1 gccagtaaag cggcagatga ctgccactca gcccacatac ggcattcctc ccatcccaca 61 gtcttagagc cagatgaggc taacaagacc acagtagccc ccttcccagg taccacactg 121 tccctggaga tccctccagc ctagccctgc tatagatctg ctctagactg tgaaatcagc 181 atgtatgtga aaaatcccag tccttgacca tgtctcccac tccacctgca agtgattgct 241 gccatctgtc ctgggtggga tgcaccgagc tagtcacggg tgtcggatca gatcttttaa 301 aaacacttgt ctcatgtaat tgagacccgc caccatcgca gatgagtgac aagagagaag 361 gctgtcaaca cttcggtaca tggtgatctt aaaaacaccc acctgcacca tcacaccaga 421 ctgtaccact agggcatcca gaacaagccc caccctaaca gtgggggcag ggagggtaca 481 gccaggctca cagcactgag agtctcaacc agctaagcta aattgcaagc tagatgcctc 541 ctgcccctgc cctcagctgc ccaggcccca gcatggagat ggcctgcac //