LOCUS       CK334270                 611 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3012E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3012E08 5', mRNA sequence.
ACCESSION   CK334270
VERSION     CK334270.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3012  row: E  column: 08
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3012E08-5"
                     /db_xref="taxon:10090"
                     /clone="H3012E08"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          156 a          134 c          168 g          153 t
ORIGIN      
        1 ggaaattacg tcattcagaa gttttttgaa tttggcagtc atgaacagaa gctggctctg
       61 gctgaacgga ttcgaggcca tgtcctatca ttggcactgc agatgtacgg ctgccgagtc
      121 atccagaaag ctttggagtt tattccctca gaccagcaga acgagatggt gcgggagcta
      181 gatggccatg tcctgaagtg tgtgaaagac cagaacggca accatgtggt tcagaagtgc
      241 attgagtgtg tacagcccca gtctttgcag tttatcatcg atgcattcaa ggggcaggtg
      301 tttgctttgt ctacgcaccc gtacggctgt agggtgatcc agaggatcct agagcactgt
      361 ctccctgacc agaccctccc catcctggag gagcttcacc agcacacaga gcagcttgtg
      421 caggatcagt atggaaatta tgtaatccaa catgtactgg agcatggtcg tcctgaggat
      481 aaaagcaaaa ttgtagcaga gatccgaggc aatgtacttg tattgagtca gcacaagttt
      541 gcaagcaatg ttgtggagaa gtgtgttact cacgcctcac gtacagagcg tgctgtgctc
      601 attgatgagg t
//