LOCUS CK334248 629 bp mRNA linear EST 12-MAY-2010 DEFINITION H3011H01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3011H01 5', mRNA sequence. ACCESSION CK334248 VERSION CK334248.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 629) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3011 row: H column: 01 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..629 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3011H01-5" /db_xref="taxon:10090" /clone="H3011H01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 140 c 176 g 161 t ORIGIN 1 gtaacccgag gtctgacaca ggtactgatt acgaagagca agcctccaag gaagaattgg 61 gagagccagt agttttcaaa aacccagtaa ccagtcaccc atgggcccag agcacaggag 121 gtgggtatcc agctcagaga gagtagatac acccttcctg ggccctcttg ttctttctag 181 gcttttggtg ttgcccagtg cactgagtga agaggttgga gtgcactgag tgttggaggc 241 ctcttgaatt tcccaatgtc ccatggtcat tgtaacaggt aaaaggctgg gtcttccctt 301 ggatctgtga ctcttggaac ttggcccatt gtattgaaag aatagccact taatatccag 361 ataatgaggg cagatgcagg ttgcgcctct tggaggaccc atccccagtt cagcagtact 421 tggaagtcag tacctgagtc gtgtggctta ggaatgtcac tgtctgtcaa ggcctgtatg 481 gtgagccaga agtatccaga gtgggccagg tgaaggttaa caagctgaca attgcttcag 541 gaagctctgg gaaaacttgg ttatcctgat gggggtccca acagcaggat cagttgtctg 601 cgtttgactt tactctgcag tctggtcct //