LOCUS CK334247 651 bp mRNA linear EST 12-MAY-2010 DEFINITION H3011G12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3011G12 5', mRNA sequence. ACCESSION CK334247 VERSION CK334247.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 651) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3011 row: G column: 12 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..651 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3011G12-5" /db_xref="taxon:10090" /clone="H3011G12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 164 a 156 c 186 g 145 t ORIGIN 1 gggagcagcc gggtagagag gagcgtggcc ttctcctctc cccgtcatgg cttgtgcccg 61 tcccctcata tcggtgtact ccgaaaaggg agagtcatct ggcaagaatg tcactttgcc 121 agctgtgttc aaagctccca ttcgaccaga tattgtgaac ttcgttcaca ccaacttgag 181 gaaaaacaac agacagccct atgccgtcag tgaattggca ggtcatcaga ccagtgctga 241 gtcttggggt actggcagag ctgtggctcg aattccaaga gttcgaggtg gtgggaccca 301 ccgatctggc cagggtgcct ttggaaatat gtgtcgtggg ggacgcatgt ttgcaccaac 361 caaaacctgg cgtcgttggc accgcagagt gaatacaacc caaaaacgat atgccatctg 421 ttctgccctg gctgcctcgg ccttaccagc tttggtgatg tctaaaggtc atcgtattga 481 ggaggttcct gagctgccgc tggtggttga agataaggtt gaaggttaca agaagaccaa 541 ggaggctgtt cagctgctca agaaactcaa agcttggaat gacatcaaaa aggtctatgc 601 ctctcagaga atgagagctg gcaagggcaa aatgagaaac cgacggcgga t //