LOCUS CK334187 637 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007H04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007H04 5', mRNA sequence. ACCESSION CK334187 VERSION CK334187.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 637) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3007 row: H column: 04 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..637 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007H04-5" /db_xref="taxon:10090" /clone="H3007H04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 166 c 160 g 155 t ORIGIN 1 gaccggctac cagtgcagag catcccaggc cccaaccttt cctggacagt tggacctctt 61 cctgagagac cccggaacgt tttctacctt acccttggga gaagagcctc tggctgaatg 121 gcagctgtgg atgacttaca gtttgaagaa tttggcgatg gagctacact gttagcagca 181 aatccagatg ctaccacgat aaacattgag gaccccagtg tatcttttgg ccaccaaccc 241 agaccccccg ggagcgtggg gcgagaagag gatgaggagc tgctggggaa caacgactct 301 gacgagaccg agttacttgc cggtcagaag cgaagctctc ccttctggac attcgaatac 361 tatcagacat tctttgatgt ggacacttac caggtctttg acagaataaa agggtccctg 421 ctgccggttc ctggaaagaa ctttgtgagg ctgtacatcc gcagcaatcc agatctctat 481 ggtccctttt ggatatgtgc cacgttggtc tttgccatag caattagtgg gaacctttct 541 aacttcctaa tccatctggg agagaagaca taccattatg tgcccgaatt ccagaaagtg 601 tctatcgcag cgactgtcat ctatgcctat gcctggc //