LOCUS CK334166 629 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007E06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007E06 5', mRNA sequence. ACCESSION CK334166 VERSION CK334166.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 629) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3007 row: E column: 06 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..629 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007E06-5" /db_xref="taxon:10090" /clone="H3007E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 188 c 155 g 131 t ORIGIN 1 tggtgacact agacagagca actccagcgt taccgctccc gctcctggtt tctcggcttc 61 tcatcgcagt caatcttgga ctttggggtt ttgctactgt cagaaggact tctttctgct 121 tcaagtgctt gacaacgcac ccctttatca gggtatcaga gcatcgccac agaatgaagc 181 tggtttccat caccctgatg ttattgggtt cactcgcttt cctaggcgcg gacactgcag 241 ggccagatac tccttcgcag ttccgaaaga agtggaataa gtgggcgcta agtcgtggga 301 agagggaact acaagcatcc agcagctacc ctacgggact cgctgatgag acgacagttc 361 ctacccagac tcttgatcca ttcctggacg agcagaacac aactggcccc ctacaagcca 421 gcaatcagag cgaagcccac attcgtgtca aacgctaccg ccagagcatg aaccagggtt 481 cccgcagcaa tggatgccgc ttcgggacct gcacatttca gaaattggcc caccagatct 541 accagctaac agacaaagac aaggacggca tggctcccag aaacaagatc agccctcaag 601 gctatggccg ccggcgccgg cgttccctg //