LOCUS CK334158 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007D04 5', mRNA sequence. ACCESSION CK334158 VERSION CK334158.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3007 row: D column: 04 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007D04-5" /db_xref="taxon:10090" /clone="H3007D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 193 c 157 g 180 t ORIGIN 1 tgaagtggca tgcttggata tcaccccatt aggggacagc aatggcctct ccccactttg 61 tgccattgga ctttggacag acatctcagc tcgtattctg aagctcccat cctttgaact 121 actacacaag gagatgctgg gtggagagat cattcctcga tccatcctga tgaccacttt 181 tgagagtagc cactacctcc tttgtgcctt gggagatggg gctcttttct actttgggct 241 caacatcgag acaggtttac tgagtgaccg taaaaaggtg actttgggca cccagcccac 301 ggtgttgagg acattccgtt ctctttctac caccaacgtc tttgcttgct ctgaccggcc 361 cactgtcatc tatagcagca atcacaagtt ggtcttctcc aatgtcaacc tcaaggaggt 421 gaactatatg tgtcctctca actcagatgg ctatcctgac agtctggcat tggccaataa 481 cagcactctc accattggca ccatcgatga gatccagaag ctccatattc gcacagttcc 541 cctctatgag tctcccagga agatctgcta tcaggaagtg tctcagtgct ttggggtcct 601 ttccagccgc attgaagtcc aagatagcag tggaggcact actgctttga ggcccagtgc 661 cagcacccag gccctgtcca gcagc //