LOCUS CK334148 625 bp mRNA linear EST 12-MAY-2010 DEFINITION H3007B08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3007B08 5', mRNA sequence. ACCESSION CK334148 VERSION CK334148.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 625) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3007 row: B column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..625 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3007B08-5" /db_xref="taxon:10090" /clone="H3007B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 142 a 190 c 172 g 121 t ORIGIN 1 gtggaagccg agccgggaac aagggaggaa cctgtaggcc gcggtgcgga gaacccaccg 61 aaggacctaa gaatctggaa cagtccaccc gagattcctt ccaggactgc cggcggcctc 121 gcgcagccag cccgggattt gcagccgacc ttctttccgg gtggaaggac ggcctttgtc 181 ccagtaacgc aggagtcgcc ccccaccccc aaccagctcg cgttcctggg tcggggcagc 241 gcaggacagg gcaataagcc tgtgcgcgca atccgcctcg ccgcccttgc tccgaagcac 301 tccagccatg aagctcaact tttctctacg gctgagagtt ttcaatctca actgctggga 361 catcccctac ctgagcaaac atagggcgga ccgcatgaag cgcttgggag actttctgaa 421 cttggaaaac tttgatctgg ctctcctgga ggaggtgtgg agtgagcagg acttccagta 481 cctaaggcaa aggctatcgc tcacctatcc agatgcacac tacttcagaa gcgggatgat 541 aggcagtggc ctctgtgtgt tctccaaaca cccaatccag gaaatcttcc agcatgtcta 601 cagtctgaat ggttacccct acatg //