LOCUS CK334115 653 bp mRNA linear EST 12-MAY-2010 DEFINITION H3006B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3006B03 5', mRNA sequence. ACCESSION CK334115 VERSION CK334115.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 653) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3006 row: B column: 03 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..653 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3006B03-5" /db_xref="taxon:10090" /clone="H3006B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 130 c 114 g 240 t ORIGIN 1 gcaaaaccct taagtatgag ggataggacc cccacccatg ggtttacata tatttggggc 61 tcattatttt ttagcatttt ctttatctcc ggatagccat ttcctggact tttgaacttc 121 ccatcttcta tgcttatgtc ccactgtgcc tgctccccca tcctctttct tgtagccttt 181 tgactcagtg gaatcgaaga tacaattttc cttagactac ctagtggagc agggaatact 241 ctgcatctca ggtaaaaaaa tgagtacact gaacaatatg taattcatac gtattttgca 301 acttacctgt gagcctgcaa ttatttgagg tttttgagca attggtgttt ctatgtgttt 361 tctaaataga gatgatatct tttttttttt cacttagaat ttagctccat ttgcttgaga 421 gagggggaaa aattcatatc tgtttctctt tgtttgcaaa taagccttag gatggctgtt 481 ttctatgctg taagtggtcc tgtcaattct gtgttttatt gagagggaga caaatggagc 541 tccaactatt tactcacttt tgcatatatt aggtttccaa ttctattcca ttctcacaat 601 ataagcatat atatccccat tccaaccatt tctgcaaatt taaaacagtg aga //