LOCUS BT020057 627 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens muscle RAS oncogene homolog mRNA, complete cds. ACCESSION BT020057 VERSION BT020057.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 627) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 627) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..627 /db_xref="H-InvDB:HIT000266797" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01552X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..627 /note="Mutations: 541:K->E;626:OK" /codon_start=1 /product="muscle RAS oncogene homolog" /protein_id="AAV38860.1" /translation="MATSAVPSDNLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDP TIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEH VDRFHQLILRVKDRESFPMILVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDP PLNVDKAFHDLVRVIRQQIPEKSQKKKKKTKWRGDRATGTHKLQCVIL" BASE COUNT 180 a 167 c 163 g 117 t ORIGIN 1 atggcaacca gcgccgtccc cagtgacaac ctccccacat acaagctggt ggtggtgggg 61 gatgggggtg tgggcaaaag tgccctcacc atccagtttt tccagaagat ctttgtgcct 121 gactatgacc ccaccattga agactcctac ctgaaacata cggagattga caatcaatgg 181 gccatcttgg acgttctgga cacagctggg caggaggaat tcagcgccat gcgggagcaa 241 tacatgcgca cgggggatgg cttcctcatc gtctactccg tcactgacaa ggccagcttt 301 gagcacgtgg accgcttcca ccagcttatc ctgcgcgtca aagacaggga gtcattcccg 361 atgatcctcg tggccaacaa ggtcgatttg atgcacttga ggaagatcac cagggagcaa 421 ggaaaagaaa tggcgaccaa acacaatatt ccgtacatag aaaccagtgc caaggaccca 481 cctctcaatg tcgacaaagc cttccatgac ctcgttagag taattaggca acagattccg 541 gaaaaaagcc agaagaagaa gaagaaaacc aaatggcggg gagaccgggc cacaggcacc 601 cacaaactgc aatgtgtgat cttgtag //