LOCUS BT020021 1191 bp mRNA linear HUM 28-OCT-2004
DEFINITION Homo sapiens Cockayne syndrome 1 (classical) mRNA, complete cds.
ACCESSION BT020021
VERSION BT020021.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1191)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1191)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1191
/db_xref="H-InvDB:HIT000266780"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH01139X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1191
/note="Mutations: 1190:OK"
/codon_start=1
/product="Cockayne syndrome 1 (classical)"
/protein_id="AAV38824.1"
/translation="MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGG
GINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSV
ETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVA
VGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRR
ASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSN
GENTLVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHY
KTVDCCVFQSNFQELYSGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSS
DEEG"
BASE COUNT 366 a 215 c 271 g 339 t
ORIGIN
1 atgctggggt ttttgtccgc acgccaaacg ggtttggagg accctcttcg ccttcggaga
61 gcagagtcaa cacggagagt tttgggactg gaattaaata aagacagaga tgttgaaaga
121 atccacggcg gtggaattaa cacccttgac attgaacctg ttgaagggag atacatgtta
181 tcaggtggtt cagatggtgt gattgtactt tatgaccttg agaactccag cagacaatct
241 tattacacat gtaaagcagt gtgttccatt ggcagagatc atcctgatgt tcacagatac
301 agtgtggaga ctgtacagtg gtatcctcat gacactggca tgttcacatc aagctcattt
361 gataaaactc tgaaagtatg ggatacaaat acattacaaa ctgcagatgt atttaatttt
421 gaggaaacag tttatagtca tcatatgtct ccagtctcca ccaagcactg tttggtagca
481 gttggtacta gaggacccaa agtacaactt tgtgacttga agtctggatc ctgttctcac
541 attctacagg gtcacagaca agaaatatta gcagtttcct ggtctccacg ttatgactat
601 atcttggcaa cagcaagtgc tgacagtaga gtaaaattat gggatgtgag aagagcatca
661 ggatgtttga ttactcttga tcaacataat gggaaaaagt cacaagctgt tgaatcagca
721 aacactgctc ataatgggaa agttaatggc ttatgtttta caagtgatgg acttcacctc
781 ctcactgttg gtacagataa tcgaatgagg ctctggaata gttccaatgg agaaaacaca
841 cttgtgaact atggaaaagt ttgtaataac agtaaaaaag gattgaaatt cactgtctcc
901 tgtggctgca gttcagaatt tgtttttgta ccatatggta gcaccattgc tgtttataca
961 gtttactcag gagaacagat aactatgctt aagggacatt ataaaactgt tgactgctgt
1021 gtatttcagt caaatttcca ggaactttat agtggtagca gagactgcaa cattctggct
1081 tgggttccat ccttatatga accagttcct gatgatgatg agactacaac aaaatcacaa
1141 ttaaatccgg cctttgaaga tgcctggagc agcagtgatg aagaaggata g
//