LOCUS BT019976 579 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens phosphomevalonate kinase mRNA, complete cds. ACCESSION BT019976 VERSION BT019976.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 579) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 579) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..579 /db_xref="H-InvDB:HIT000266756" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01516X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..579 /note="Mutations: 147:OK;373:V->M" /codon_start=1 /product="phosphomevalonate kinase" /protein_id="AAV38779.1" /translation="MAPLGGAPRLVLLFSGKRKSGKDFVTEALQSRLGADVCAVLRLS GPLKEQYAQEHGLNFQRLLDTSTYKEAFRKDMIRWGEEKRQADPGFFCRKIVEGISQP IWLVSDTRRVSDIQWFREAYGAMTQTVRVVALEQSRQQRGWVFTPGVDDAESECGLDN FGDFDWVIENHGVEQRLEEQLENLIEFIRSRL" BASE COUNT 123 a 144 c 198 g 114 t ORIGIN 1 atggccccgc tgggaggcgc cccgcggctg gtactgctgt tcagcggcaa gaggaaatcc 61 gggaaggact tcgtgaccga ggcgctgcag agcagacttg gagctgatgt ctgtgctgtc 121 ctccggctct ctggtccact caaggagcag tatgctcagg agcatggctt gaacttccag 181 agactcctgg acaccagcac ctacaaggag gcctttcgga aggacatgat ccgctgggga 241 gaggagaaac gccaggctga cccaggcttc ttttgcagga agattgtgga gggcatctcc 301 cagcccatct ggctggtgag tgacacacgg agagtgtctg acatccagtg gtttcgggag 361 gcctatgggg ccatgacgca gacggtccgc gttgtagcgt tggagcagag ccgacagcag 421 cggggctggg tgttcacgcc aggggtggac gatgctgagt cagaatgtgg cctggacaac 481 ttcggggact ttgactgggt catcgagaac catggagttg aacagcgcct ggaggagcag 541 ttggagaacc tgatagaatt tatccgctcc agactttag //