LOCUS       BT019976                 579 bp    mRNA    linear   HUM 28-OCT-2004
DEFINITION  Homo sapiens phosphomevalonate kinase mRNA, complete cds.
ACCESSION   BT019976
VERSION     BT019976.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 579)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
            circle, Palo Alto, California 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..579
                     /db_xref="H-InvDB:HIT000266756"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH01516X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..579
                     /note="Mutations: 147:OK;373:V->M"
                     /codon_start=1
                     /product="phosphomevalonate kinase"
                     /protein_id="AAV38779.1"
                     /translation="MAPLGGAPRLVLLFSGKRKSGKDFVTEALQSRLGADVCAVLRLS
                     GPLKEQYAQEHGLNFQRLLDTSTYKEAFRKDMIRWGEEKRQADPGFFCRKIVEGISQP
                     IWLVSDTRRVSDIQWFREAYGAMTQTVRVVALEQSRQQRGWVFTPGVDDAESECGLDN
                     FGDFDWVIENHGVEQRLEEQLENLIEFIRSRL"
BASE COUNT          123 a          144 c          198 g          114 t
ORIGIN      
        1 atggccccgc tgggaggcgc cccgcggctg gtactgctgt tcagcggcaa gaggaaatcc
       61 gggaaggact tcgtgaccga ggcgctgcag agcagacttg gagctgatgt ctgtgctgtc
      121 ctccggctct ctggtccact caaggagcag tatgctcagg agcatggctt gaacttccag
      181 agactcctgg acaccagcac ctacaaggag gcctttcgga aggacatgat ccgctgggga
      241 gaggagaaac gccaggctga cccaggcttc ttttgcagga agattgtgga gggcatctcc
      301 cagcccatct ggctggtgag tgacacacgg agagtgtctg acatccagtg gtttcgggag
      361 gcctatgggg ccatgacgca gacggtccgc gttgtagcgt tggagcagag ccgacagcag
      421 cggggctggg tgttcacgcc aggggtggac gatgctgagt cagaatgtgg cctggacaac
      481 ttcggggact ttgactgggt catcgagaac catggagttg aacagcgcct ggaggagcag
      541 ttggagaacc tgatagaatt tatccgctcc agactttag
//