LOCUS BT019962 564 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens DNA-dependent protein kinase catalytic subunit-interacting protein 2 mRNA, complete cds. ACCESSION BT019962 VERSION BT019962.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 564) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 564) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..564 /db_xref="H-InvDB:HIT000266748" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01510X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..564 /note="Mutations: 430:E->K;448:D->N;477:OK;495:OK;563:OK" /codon_start=1 /product="DNA-dependent protein kinase catalytic subunit-interacting protein 2" /protein_id="AAV38765.1" /translation="MGNKQTIFTEEQLDNYQDCTFFNKKDILKLHSRFYELAPNLVPM DYRKSPIVHVPMSLIIQMPELRENPFKERIVAAFSEDGEGNLTFNDFVDMFSVLCESA PRELKANYAFKIYDFNTDNFICKEDLELTLARLTKSELDEEKVVLVCNKVIEEADLDG DGKLGFADFEDMIAKAPDFLSTFHIRI" BASE COUNT 139 a 155 c 149 g 121 t ORIGIN 1 atggggaaca agcagaccat cttcaccgaa gagcagctag acaactacca ggactgcacc 61 ttcttcaata agaaggacat cctcaagctg cattcgcgat tctatgagct ggcccccaac 121 ctcgtcccaa tggactacag gaagagcccc atcgtccacg tgcccatgag cctcatcatc 181 cagatgccag agctccggga gaatcccttc aaagaaagga tcgtggcggc gttttccgag 241 gatggtgagg ggaacctcac tttcaacgac tttgtggaca tgttttccgt gctctgcgag 301 tcggctcccc gagagctcaa ggcaaactat gccttcaaga tctatgactt caacactgac 361 aacttcatct gcaaggagga cctggagctg acgctggccc ggctcactaa gtcagagctg 421 gatgaggaga aggtggtgct tgtgtgcaac aaggtcattg aggaggctga cttggacggt 481 gacggcaagc tgggttttgc tgacttcgag gacatgattg ccaaggcccc tgacttcctc 541 agcactttcc acatccggat ctag //