LOCUS BT019775 1065 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 mRNA, complete cds. ACCESSION BT019775 VERSION BT019775.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1065) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 1065) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..1065 /db_xref="H-InvDB:HIT000266651" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01268X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..1065 /note="Mutations: 117:OK;655:A->T;730:H->Y;745:L->M;819:OK;863:Q->P" /codon_start=1 /product="guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1" /protein_id="AAV38580.1" /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGES GKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARAD DARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLD RIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV TAIIFCVALSDYDLVLAEDEEMNRMYESMKMFDSICNNKWFTDTSIILFLNKKDLFEE KIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQF VFDAVTDVIIKNNLKDCGLF" BASE COUNT 345 a 192 c 259 g 269 t ORIGIN 1 atgggctgca cgctgagcgc cgaggacaag gcggcggtgg agcggagtaa gatgatcgac 61 cgcaacctcc gtgaggacgg cgagaaggcg gcgcgcgagg tcaagctgct gctgcttggt 121 gctggtgaat ctggtaaaag tacaattgtg aagcagatga aaattatcca tgaagctggt 181 tattcagaag aggagtgtaa acaatacaaa gcagtggtct acagtaacac catccagtca 241 attattgcta tcattagggc tatggggagg ttgaagatag actttggtga ctcagcccgg 301 gcggatgatg cacgccaact ctttgtgcta gctggagctg ctgaagaagg ctttatgact 361 gcagaacttg ctggagttat aaagagattg tggaaagata gtggtgtaca agcctgtttc 421 aacagatccc gagagtacca gcttaatgat tctgcagcat actatttgaa tgacttggac 481 agaatagctc aaccaaatta catcccgact caacaagatg ttctcagaac tagagtgaaa 541 actacaggaa ttgttgaaac ccattttact ttcaaagatc ttcattttaa aatgtttgat 601 gtgggaggtc agagatctga gcggaagaag tggattcatt gcttcgaagg agtgacggcg 661 atcatcttct gtgtagcact gagtgactac gacctggttc tagctgaaga tgaagaaatg 721 aaccgaatgt atgaaagcat gaaaatgttt gacagcatat gtaacaacaa gtggtttaca 781 gatacatcca ttatactttt tctaaacaag aaggatctct ttgaagaaaa aatcaaaaag 841 agccctctca ctatatgcta tccagaatat gcaggatcaa acacatatga agaggcagct 901 gcatatattc aatgtcagtt tgaagacctc aataaaagaa aggacacaaa ggaaatatac 961 acccacttca catgtgccac agatactaag aatgtgcagt ttgtttttga tgctgtaaca 1021 gatgtcatca taaaaaataa tctaaaagat tgtggtctct tttag //