LOCUS       BT019775                1065 bp    mRNA    linear   HUM 28-OCT-2004
DEFINITION  Homo sapiens guanine nucleotide binding protein (G protein), alpha
            inhibiting activity polypeptide 1 mRNA, complete cds.
ACCESSION   BT019775
VERSION     BT019775.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
            circle, Palo Alto, California 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..1065
                     /db_xref="H-InvDB:HIT000266651"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH01268X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..1065
                     /note="Mutations:
                     117:OK;655:A->T;730:H->Y;745:L->M;819:OK;863:Q->P"
                     /codon_start=1
                     /product="guanine nucleotide binding protein (G protein),
                     alpha inhibiting activity polypeptide 1"
                     /protein_id="AAV38580.1"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGES
                     GKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARAD
                     DARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLD
                     RIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV
                     TAIIFCVALSDYDLVLAEDEEMNRMYESMKMFDSICNNKWFTDTSIILFLNKKDLFEE
                     KIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQF
                     VFDAVTDVIIKNNLKDCGLF"
BASE COUNT          345 a          192 c          259 g          269 t
ORIGIN      
        1 atgggctgca cgctgagcgc cgaggacaag gcggcggtgg agcggagtaa gatgatcgac
       61 cgcaacctcc gtgaggacgg cgagaaggcg gcgcgcgagg tcaagctgct gctgcttggt
      121 gctggtgaat ctggtaaaag tacaattgtg aagcagatga aaattatcca tgaagctggt
      181 tattcagaag aggagtgtaa acaatacaaa gcagtggtct acagtaacac catccagtca
      241 attattgcta tcattagggc tatggggagg ttgaagatag actttggtga ctcagcccgg
      301 gcggatgatg cacgccaact ctttgtgcta gctggagctg ctgaagaagg ctttatgact
      361 gcagaacttg ctggagttat aaagagattg tggaaagata gtggtgtaca agcctgtttc
      421 aacagatccc gagagtacca gcttaatgat tctgcagcat actatttgaa tgacttggac
      481 agaatagctc aaccaaatta catcccgact caacaagatg ttctcagaac tagagtgaaa
      541 actacaggaa ttgttgaaac ccattttact ttcaaagatc ttcattttaa aatgtttgat
      601 gtgggaggtc agagatctga gcggaagaag tggattcatt gcttcgaagg agtgacggcg
      661 atcatcttct gtgtagcact gagtgactac gacctggttc tagctgaaga tgaagaaatg
      721 aaccgaatgt atgaaagcat gaaaatgttt gacagcatat gtaacaacaa gtggtttaca
      781 gatacatcca ttatactttt tctaaacaag aaggatctct ttgaagaaaa aatcaaaaag
      841 agccctctca ctatatgcta tccagaatat gcaggatcaa acacatatga agaggcagct
      901 gcatatattc aatgtcagtt tgaagacctc aataaaagaa aggacacaaa ggaaatatac
      961 acccacttca catgtgccac agatactaag aatgtgcagt ttgtttttga tgctgtaaca
     1021 gatgtcatca taaaaaataa tctaaaagat tgtggtctct tttag
//