LOCUS       BT019744                 984 bp    mRNA    linear   HUM 28-OCT-2004
DEFINITION  Homo sapiens protein phosphatase 1, catalytic subunit, beta isoform
            mRNA, complete cds.
ACCESSION   BT019744
VERSION     BT019744.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 984)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
            circle, Palo Alto, California 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..984
                     /db_xref="H-InvDB:HIT000266636"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH01301X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..984
                     /note="Mutations: 152:L->P;201:OK;983:OK"
                     /codon_start=1
                     /product="protein phosphatase 1, catalytic subunit, beta
                     isoform"
                     /protein_id="AAV38549.1"
                     /translation="MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREI
                     FLSQPIPLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLET
                     ICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPI
                     AAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGEN
                     DRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFD
                     NAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR"
BASE COUNT          275 a          168 c          245 g          296 t
ORIGIN      
        1 atggcggacg gggagctgaa cgtggacagc ctcatcaccc ggctgctgga ggtacgagga
       61 tgtcgtccag gaaagattgt gcagatgact gaagcagaag ttcgaggctt atgtatcaag
      121 tctcgggaga tctttctcag ccagcctatt cctttggaat tggaagcacc gctgaaaatt
      181 tgtggagata ttcatggaca gtatacagat ttactgagat tatttgaata tggaggtttc
      241 ccaccagaag ccaactatct tttcttagga gattatgtgg acagaggaaa gcagtctttg
      301 gaaaccattt gtttgctatt ggcttataaa atcaaatatc cagagaactt ctttctctta
      361 agaggaaacc atgagtgtgc tagcatcaat cgcatttatg gattctatga tgaatgcaaa
      421 cgaagattta atattaaatt gtggaagacc ttcactgatt gttttaactg tctgcctata
      481 gcagccattg tggatgagaa gatcttctgt tgtcatggag gattgtcacc agacctgcaa
      541 tctatggagc agattcggag aattatgaga cctactgatg tccctgatac aggtttgctc
      601 tgtgatttgc tatggtctga tccagataag gatgtgcaag gctggggaga aaatgatcgt
      661 ggtgtttcct ttacttttgg agctgatgta gtcagtaaat ttctgaatcg tcatgattta
      721 gatttgattt gtcgagctca tcaggtggtg gaagatggat atgaattttt tgctaaacga
      781 cagttggtaa ccttattttc agccccaaat tactgtggcg agtttgataa tgctggtgga
      841 atgatgagtg tggatgaaac tttgatgtgt tcatttcaga tattgaaacc atctgaaaag
      901 aaagctaaat accagtatgg tggactgaat tctggacgtc ctgtcactcc acctcgaaca
      961 gctaatccgc cgaagaaaag gtag
//