LOCUS BT019744 984 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens protein phosphatase 1, catalytic subunit, beta isoform mRNA, complete cds. ACCESSION BT019744 VERSION BT019744.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 984) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 984) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..984 /db_xref="H-InvDB:HIT000266636" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01301X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..984 /note="Mutations: 152:L->P;201:OK;983:OK" /codon_start=1 /product="protein phosphatase 1, catalytic subunit, beta isoform" /protein_id="AAV38549.1" /translation="MADGELNVDSLITRLLEVRGCRPGKIVQMTEAEVRGLCIKSREI FLSQPIPLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLET ICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPI AAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGEN DRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFD NAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPPRTANPPKKR" BASE COUNT 275 a 168 c 245 g 296 t ORIGIN 1 atggcggacg gggagctgaa cgtggacagc ctcatcaccc ggctgctgga ggtacgagga 61 tgtcgtccag gaaagattgt gcagatgact gaagcagaag ttcgaggctt atgtatcaag 121 tctcgggaga tctttctcag ccagcctatt cctttggaat tggaagcacc gctgaaaatt 181 tgtggagata ttcatggaca gtatacagat ttactgagat tatttgaata tggaggtttc 241 ccaccagaag ccaactatct tttcttagga gattatgtgg acagaggaaa gcagtctttg 301 gaaaccattt gtttgctatt ggcttataaa atcaaatatc cagagaactt ctttctctta 361 agaggaaacc atgagtgtgc tagcatcaat cgcatttatg gattctatga tgaatgcaaa 421 cgaagattta atattaaatt gtggaagacc ttcactgatt gttttaactg tctgcctata 481 gcagccattg tggatgagaa gatcttctgt tgtcatggag gattgtcacc agacctgcaa 541 tctatggagc agattcggag aattatgaga cctactgatg tccctgatac aggtttgctc 601 tgtgatttgc tatggtctga tccagataag gatgtgcaag gctggggaga aaatgatcgt 661 ggtgtttcct ttacttttgg agctgatgta gtcagtaaat ttctgaatcg tcatgattta 721 gatttgattt gtcgagctca tcaggtggtg gaagatggat atgaattttt tgctaaacga 781 cagttggtaa ccttattttc agccccaaat tactgtggcg agtttgataa tgctggtgga 841 atgatgagtg tggatgaaac tttgatgtgt tcatttcaga tattgaaacc atctgaaaag 901 aaagctaaat accagtatgg tggactgaat tctggacgtc ctgtcactcc acctcgaaca 961 gctaatccgc cgaagaaaag gtag //