LOCUS       BT019717                 726 bp    mRNA    linear   HUM 28-OCT-2004
DEFINITION  Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 5
            mRNA, complete cds.
ACCESSION   BT019717
VERSION     BT019717.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 726)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
            circle, Palo Alto, California 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..726
                     /db_xref="H-InvDB:HIT000266623"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH01312X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..726
                     /note="Mutations: 101:G->A"
                     /codon_start=1
                     /product="proteasome (prosome, macropain) subunit, alpha
                     type, 5"
                     /protein_id="AAV38522.1"
                     /translation="MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLASTAIGIQTSE
                     GVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFT
                     YNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSG
                     TFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELA
                     TVQPGQNFHMFTKEELEEVIKDI"
BASE COUNT          207 a          149 c          185 g          185 t
ORIGIN      
        1 atgtttctta cccggtctga gtacgacagg ggcgtgaata ctttttctcc cgaaggaaga
       61 ttatttcaag tggaatatgc cattgaggct atcaagcttg cttctacagc cattgggatc
      121 cagacatcag agggtgtgtg cctagctgtg gagaagagaa ttacttcccc actgatggag
      181 cccagcagca ttgagaaaat tgtagagatt gatgctcaca taggttgtgc catgagtggg
      241 ctaattgctg atgctaagac tttaattgat aaagccagag tggagacaca gaaccactgg
      301 ttcacctaca atgagacaat gacagtggag agtgtgaccc aagctgtgtc caatctggct
      361 ttgcagtttg gagaagaaga tgcagatcca ggtgccatgt ctcgtccctt tggagtagca
      421 ttattatttg gaggagttga tgagaaagga ccccagctgt ttcatatgga cccatctggg
      481 acctttgtac agtgtgatgc tcgagcaatt ggctctgctt cagagggtgc ccagagctcc
      541 ttgcaagaag tttaccacaa gtctatgact ttgaaagaag ccatcaagtc ttcactcatc
      601 atcctcaaac aagtaatgga ggagaagctg aatgcaacaa acattgagct agccacagtg
      661 cagcctggcc agaatttcca catgttcaca aaggaagaac ttgaagaggt tatcaaggac
      721 atttag
//