LOCUS BT019717 726 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 5 mRNA, complete cds. ACCESSION BT019717 VERSION BT019717.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 726) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 726) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..726 /db_xref="H-InvDB:HIT000266623" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01312X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..726 /note="Mutations: 101:G->A" /codon_start=1 /product="proteasome (prosome, macropain) subunit, alpha type, 5" /protein_id="AAV38522.1" /translation="MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLASTAIGIQTSE GVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFT YNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSG TFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELA TVQPGQNFHMFTKEELEEVIKDI" BASE COUNT 207 a 149 c 185 g 185 t ORIGIN 1 atgtttctta cccggtctga gtacgacagg ggcgtgaata ctttttctcc cgaaggaaga 61 ttatttcaag tggaatatgc cattgaggct atcaagcttg cttctacagc cattgggatc 121 cagacatcag agggtgtgtg cctagctgtg gagaagagaa ttacttcccc actgatggag 181 cccagcagca ttgagaaaat tgtagagatt gatgctcaca taggttgtgc catgagtggg 241 ctaattgctg atgctaagac tttaattgat aaagccagag tggagacaca gaaccactgg 301 ttcacctaca atgagacaat gacagtggag agtgtgaccc aagctgtgtc caatctggct 361 ttgcagtttg gagaagaaga tgcagatcca ggtgccatgt ctcgtccctt tggagtagca 421 ttattatttg gaggagttga tgagaaagga ccccagctgt ttcatatgga cccatctggg 481 acctttgtac agtgtgatgc tcgagcaatt ggctctgctt cagagggtgc ccagagctcc 541 ttgcaagaag tttaccacaa gtctatgact ttgaaagaag ccatcaagtc ttcactcatc 601 atcctcaaac aagtaatgga ggagaagctg aatgcaacaa acattgagct agccacagtg 661 cagcctggcc agaatttcca catgttcaca aaggaagaac ttgaagaggt tatcaaggac 721 atttag //