LOCUS       BT019553                 597 bp    mRNA    linear   HUM 28-OCT-2004
DEFINITION  Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) mRNA,
            complete cds.
ACCESSION   BT019553
VERSION     BT019553.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 597)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow
            circle, Palo Alto, California 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..597
                     /db_xref="H-InvDB:HIT000266550"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH01404X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..597
                     /note="Mutations: 326:V->G"
                     /codon_start=1
                     /product="cyclin-dependent kinase inhibitor 1B (p27,
                     Kip1)"
                     /protein_id="AAV38360.1"
                     /translation="MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRD
                     LEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVP
                     AQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDS
                     STQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT"
BASE COUNT          160 a          164 c          187 g           86 t
ORIGIN      
        1 atgtcaaacg tgcgagtgtc taacgggagc cctagcctgg agcggatgga cgccaggcag
       61 gcggagcacc ccaagccctc ggcctgcagg aacctcttcg gcccggtgga ccacgaagag
      121 ttaacccggg acttggagaa gcactgcaga gacatggaag aggcgagcca gcgcaagtgg
      181 aatttcgatt ttcagaatca caaaccccta gagggcaagt acgagtggca agaggtggag
      241 aagggcagct tgcccgagtt ctactacaga cccccgcggc cccccaaagg tgcctgcaag
      301 gtgccggcgc aggagagcca ggatggcagc gggagccgcc cggcggcgcc tttaattggg
      361 gctccggcta actctgagga cacgcatttg gtggacccaa agactgatcc gtcggacagc
      421 cagacggggt tagcggagca atgcgcagga ataaggaagc gacctgcaac cgacgattct
      481 tctactcaaa acaaaagagc caacagaaca gaagaaaatg tttcagacgg ttccccaaat
      541 gccggttctg tggagcagac gcccaagaag cctggcctca gaagacgtca aacgtag
//