LOCUS BT019553 597 bp mRNA linear HUM 28-OCT-2004 DEFINITION Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) mRNA, complete cds. ACCESSION BT019553 VERSION BT019553.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 597) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 597) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (28-OCT-2004) BD Biosciences Clontech, 1020 East Meadow circle, Palo Alto, California 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..597 /db_xref="H-InvDB:HIT000266550" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH01404X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..597 /note="Mutations: 326:V->G" /codon_start=1 /product="cyclin-dependent kinase inhibitor 1B (p27, Kip1)" /protein_id="AAV38360.1" /translation="MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRD LEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVP AQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDS STQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT" BASE COUNT 160 a 164 c 187 g 86 t ORIGIN 1 atgtcaaacg tgcgagtgtc taacgggagc cctagcctgg agcggatgga cgccaggcag 61 gcggagcacc ccaagccctc ggcctgcagg aacctcttcg gcccggtgga ccacgaagag 121 ttaacccggg acttggagaa gcactgcaga gacatggaag aggcgagcca gcgcaagtgg 181 aatttcgatt ttcagaatca caaaccccta gagggcaagt acgagtggca agaggtggag 241 aagggcagct tgcccgagtt ctactacaga cccccgcggc cccccaaagg tgcctgcaag 301 gtgccggcgc aggagagcca ggatggcagc gggagccgcc cggcggcgcc tttaattggg 361 gctccggcta actctgagga cacgcatttg gtggacccaa agactgatcc gtcggacagc 421 cagacggggt tagcggagca atgcgcagga ataaggaagc gacctgcaac cgacgattct 481 tctactcaaa acaaaagagc caacagaaca gaagaaaatg tttcagacgg ttccccaaat 541 gccggttctg tggagcagac gcccaagaag cctggcctca gaagacgtca aacgtag //