LOCUS BT009913 2247 bp mRNA linear HUM 02-AUG-2003
DEFINITION Homo sapiens cysteinyl-tRNA synthetase mRNA, complete cds.
ACCESSION BT009913
VERSION BT009913.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2247)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (17-JUL-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
REFERENCE 2 (bases 1 to 2247)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2247
/db_xref="H-InvDB:HIT000266436"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00464X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2247
/codon_start=1
/product="cysteinyl-tRNA synthetase"
/protein_id="AAP88915.1"
/translation="MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIP
QDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKI
IKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQ
LATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKF
WEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFA
SSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWP
CPWGKGRPGWHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVR
YFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSARQLRLAFLMHSWKDTLDYSSNTME
SALQYEKFLNEFFLNVKDILRAPVDITGQFEKWGEEEAELNKNFYDKKTAIHKALCDN
VDTRTVMEEMRALVSQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSS
LGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPE
LGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEAAKLAK
MKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQ
MAQNGSFQ"
BASE COUNT 612 a 550 c 623 g 462 t
ORIGIN
1 atggcagatt cctccgggca gcagggcaaa ggccggcgtg tgcagcccca gtggtcccct
61 cctgctggga cccagccatg cagactccac ctttacaaca gcctcaccag gaacaaggaa
121 gtgttcatac ctcaagatgg gaaaaaggtg acgtggtatt gctgtgggcc aaccgtctat
181 gacgcatctc acatggggca cgccaggtcc tacatctctt ttgatatctt gagaagagtg
241 ttgaaggatt acttcaaatt tgatgtcttt tattgcatga acattacgga tattgatgac
301 aagatcatca agagggcccg gcagaaccac ctgttcgagc agtatcggga gaagaggcct
361 gaagcggcac agctcttgga ggatgttcag gccgccctga agccattttc agtaaaatta
421 aatgagacca cggatcccga taaaaagcag atgctcgaac ggattcagca cgcagtgcag
481 cttgccacag agccacttga gaaagctgtg cagtccagac tcacgggaga ggaagtcaac
541 agctgtgtgg aggtgttgct ggaagaagcc aaggatttgc tctctgactg gctggattct
601 acacttggct gtgatgtcac tgacaattcc atcttctcca agctgcccaa gttctgggag
661 ggggacttcc acagagacat ggaagctctg aatgttctcc ctccagatgt cttaacccgg
721 gttagtgagt atgtgccaga aattgtgaac tttgtccaga agattgtgga caacggttac
781 ggctatgtct ccaatgggtc tgtctacttt gatacagcga agtttgcttc tagcgagaag
841 cactcctatg ggaagctggt gcctgaggcc gttggagatc agaaagccct tcaagaaggg
901 gaaggtgacc tgagcatctc tgcagaccgc ctgagtgaga agcgctctcc caacgacttt
961 gccttatgga aggcctctaa gcccggagaa ccgtcctggc cgtgcccttg gggaaagggt
1021 cgtccgggct ggcatatcga gtgctcggcc atggcaggca ccctcctagg ggcttcgatg
1081 gacattcacg gaggtgggtt cgacctccgg ttcccccacc atgacaatga gctggcacag
1141 tcggaggcct actttgaaaa cgactgctgg gtcaggtact tcctgcacac aggccacctg
1201 accattgcag gctgcaaaat gtcaaagtca ctaaaaaact tcatcaccat taaagatgcc
1261 ttgaaaaagc actcagcacg gcagttgcgg ctggccttcc tcatgcactc gtggaaggac
1321 accctggact actccagcaa caccatggag tcagcgcttc aatatgagaa gttcttgaat
1381 gagtttttct taaatgtgaa agatatcctt cgcgctcctg ttgacatcac tggtcagttt
1441 gagaagtggg gagaagaaga agcagaactg aataagaact tttatgacaa gaagacagca
1501 attcacaaag ccctctgtga caatgttgac acccgcaccg tcatggaaga gatgcgggcc
1561 ttggtcagtc agtgcaacct ctatatggca gcccggaaag ccgtgaggaa gaggcccaac
1621 caggctctgc tggagaacat cgccctgtac ctcacccata tgctgaagat ctttggggcc
1681 gtagaagagg acagctccct gggattcccg gtcggagggc ctggaaccag cctcagtctc
1741 gaggccacag tcatgcccta ccttcaggtg ttatcagaat tccgagaagg agtgcggaag
1801 attgcccgag agcaaaaagt ccctgagatt ctgcagctca gcgatgccct gcgggacaac
1861 atcctgcccg agcttggggt gcggtttgaa gaccacgaag gactgcccac agtggtgaaa
1921 ctggtagaca gaaacacctt attaaaagag agagaagaaa agagacgggt tgaagaggag
1981 aagaggaaga agaaagagga ggcggcccgg aggaaacagg aacaagaagc agcaaagctg
2041 gccaagatga agattccccc cagtgagatg ttcttgtcag aaaccgacaa atactccaag
2101 tttgatgaaa atggtctgcc cacacatgac atggagggca aagagctcag caaagggcaa
2161 gccaagaagc tgaagaagct cttcgaggct caggagaagc tctacaagga atatctgcag
2221 atggcccaga atggaagctt ccagtag
//