LOCUS BT009797 2631 bp mRNA linear HUM 02-AUG-2003
DEFINITION Homo sapiens karyopherin (importin) beta 1 mRNA, complete cds.
ACCESSION BT009797
VERSION BT009797.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2631)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (17-JUL-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
REFERENCE 2 (bases 1 to 2631)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2631
/db_xref="H-InvDB:HIT000266359"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH01127X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2631
/codon_start=1
/product="karyopherin (importin) beta 1"
/protein_id="AAP88799.1"
/translation="MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVL
ANPGNSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTE
TYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQD
IDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFI
MQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVA
LQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQD
ENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGC
ILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLA
PLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQ
KLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMES
HIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGV
QEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQ
SNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQ
QASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFIL
SFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTN
KAKTLATWATKELRKLKNQA"
BASE COUNT 745 a 560 c 674 g 652 t
ORIGIN
1 atggagctga tcaccattct cgagaagacc gtgtctcccg atcggctgga gctggaagcg
61 gcgcagaagt tcctggagcg tgcggccgtg gagaacctgc ccactttcct tgtggaactg
121 tccagagtgc tggcaaatcc aggaaacagt caggttgcca gagttgcagc tggtctacaa
181 atcaagaact ctttgacatc taaagatcca gatatcaagg cacaatatca gcagaggtgg
241 cttgctattg atgctaatgc tcgacgagaa gtcaagaact atgttttgca gacattgggt
301 acagaaactt accggcctag ttctgcctca cagtgtgtgg ctggtattgc ttgtgcagag
361 atcccagtaa accagtggcc agaactcatt cctcagctgg tggccaatgt cacaaacccc
421 aacagcacag agcacatgaa ggagtcgaca ttggaagcca tcggttatat ttgccaagat
481 atagacccag agcagctaca agataaatcc aatgagattc tgactgccat aatccagggg
541 atgaggaaag aagagcctag taataatgtg aagctagctg ctacgaatgc actcctgaac
601 tcattggagt tcaccaaagc aaactttgat aaagagtctg aaaggcactt tattatgcag
661 gtggtctgtg aagccacaca gtgtccagat acgagggtac gagtggctgc tttacagaat
721 ctggtgaaga taatgtcctt atattatcag tacatggaga catatatggg tcctgctctt
781 tttgcaatca caatcgaagc aatgaaaagt gacattgatg aggtggcttt acaagggata
841 gaattctggt ccaatgtctg tgatgaggaa atggatttgg ccattgaagc ttcagaggca
901 gcagaacaag gacggccccc tgagcacacc agcaagtttt atgcgaaggg agcactacag
961 tatctggttc caatcctcac acagacacta actaaacagg acgaaaatga tgatgacgat
1021 gactggaacc cctgcaaagc agcaggggtg tgcctcatgc ttctggccac ctgctgtgaa
1081 gatgacattg tcccacatgt cctccccttc attaaagaac acatcaagaa cccagattgg
1141 cggtaccggg atgcagcagt gatggctttt ggttgtatct tggaaggacc agagcccagt
1201 cagctcaaac cactagttat acaggctatg cccaccctaa tagaattaat gaaagacccc
1261 agtgtagttg ttcgagatac agctgcatgg actgtaggca gaatttgtga gctgcttcct
1321 gaagctgcca tcaatgatgt ctacttggct cccctgctac agtgtctgat tgagggtctc
1381 agtgctgaac ccagagtggc ttcaaatgtg tgctgggctt tctccagtct ggctgaagct
1441 gcttatgaag ctgcagacgt tgctgatgat caggaagaac cagctactta ctgcttatct
1501 tcttcatttg aactcatagt tcagaagctc ctagagacta cagacagacc tgatggacac
1561 cagaacaacc tgaggagttc tgcatatgaa tctctgatgg aaattgtgaa aaacagtgcc
1621 aaggattgtt atcctgctgt ccagaaaacg actttggtca tcatggaacg actgcaacag
1681 gttcttcaga tggagtcaca tatccagagc acatccgata gaatccagtt caatgacctt
1741 cagtctttac tctgtgcaac tcttcagaat gttcttcgga aagtgcaaca tcaagatgct
1801 ttgcagatct ctgatgtggt tatggcctcc ctgttaagga tgttccaaag cacagctggg
1861 tctgggggag tacaagagga tgccctgatg gcagttagca cactggtgga agtgttgggt
1921 ggtgaattcc tcaagtacat ggaggccttt aaacccttcc tgggcattgg attaaaaaat
1981 tatgctgaat accaggtttg tttggcagct gtgggcttag tgggagactt gtgccgtgcc
2041 ctgcaatcca acatcatacc tttctgtgac gaggtgatgc agctgcttct ggaaaatttg
2101 gggaatgaga acgtccacag gtctgtgaag ccgcagattc tgtcagtgtt tggtgatatt
2161 gcccttgcta ttggaggaga gtttaaaaaa tacttagagg ttgtattgaa tactcttcag
2221 caggcctccc aagcccaggt ggacaagtca gactatgaca tggtggatta tctgaatgag
2281 ctaagggaaa gctgcttgga agcctatact ggaatcgtcc agggattaaa gggggatcag
2341 gagaacgtac acccggatgt gatgctggta caacccagag tagaatttat tctgtctttc
2401 attgaccaca ttgctggaga tgaggatcac acagatggag tagtagcttg tgctgctgga
2461 ctaatagggg acttatgtac agcatttggg aaggatgtac tgaaattagt agaagctagg
2521 ccaatgatcc atgaattgtt aactgaaggg cggagatcga agactaacaa agcaaaaacc
2581 cttgctacat gggcaacaaa agaactgagg aaactgaaga accaagctta g
//