LOCUS BT009795 1002 bp mRNA linear HUM 02-AUG-2003
DEFINITION Homo sapiens eukaryotic translation initiation factor 2, subunit 2
beta, 38kDa mRNA, complete cds.
ACCESSION BT009795
VERSION BT009795.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1002)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (17-JUL-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
REFERENCE 2 (bases 1 to 1002)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1002
/db_xref="H-InvDB:HIT000266357"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH01125X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1002
/codon_start=1
/product="eukaryotic translation initiation factor 2,
subunit 2 beta, 38kDa"
/protein_id="AAP88797.1"
/translation="MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEV
EPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLK
IESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFS
NQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTS
FVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIK
EYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN"
BASE COUNT 373 a 174 c 230 g 225 t
ORIGIN
1 atgtctgggg acgagatgat ttttgatcct actatgagca agaagaaaaa gaagaagaag
61 aagcctttta tgttagatga ggaaggggat acccaaacag aggaaaccca gccttcagaa
121 acaaaagaag tggagccaga gccaactgag gacaaggatt tggaagctga tgaagaggac
181 actaggaaaa aagatgcttc tgatgatcta gatgacttga acttctttaa tcaaaagaaa
241 aagaagaaaa aaactaaaaa gatatttgat attgatgaag ctgaagaagg tgtaaaggat
301 cttaagattg aaagtgatgt tcaagaacca actgaaccag aggatgacct tgacattatg
361 cttggcaata aaaagaagaa aaagaagaat gttaagttcc cagatgagga tgaaatacta
421 gagaaagatg aagctctaga agatgaagac aacaaaaaag atgatggtat ctcattcagt
481 aatcagacag gccctgcttg ggcaggctca gaaagagact acacatacga ggagctgctg
541 aatcgagtgt tcaacatcat gagggaaaag aatccagata tggttgctgg ggagaaaagg
601 aaatttgtca tgaaacctcc acaagtcgtc cgagtaggaa ccaagaaaac ttcttttgtc
661 aactttacag atatctgtaa actattacat cgtcagccca aacatctcct tgcatttttg
721 ttggctgaat tgggtacaag tggttctata gatggtaata accaacttgt aatcaaagga
781 agattccaac agaaacagat agaaaatgtc ttgagaagat atatcaagga atatgtcact
841 tgtcacacat gccgatcacc ggacacaatc ctgcagaagg acacacgact ctatttccta
901 cagtgcgaaa cttgtcattc tagatgttct gttgccagta tcaaaaccgg cttccaggct
961 gtcacgggca agcgagcaca gctccgtgcc aaagctaact ag
//