LOCUS BT009783 2004 bp mRNA linear HUM 02-AUG-2003
DEFINITION Homo sapiens calpastatin mRNA, complete cds.
ACCESSION BT009783
VERSION BT009783.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2004)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (17-JUL-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
REFERENCE 2 (bases 1 to 2004)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (02-AUG-2003) BD Biosciences Clontech, 1020 East Meadow
circle, Palo Alto, California 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2004
/db_xref="H-InvDB:HIT000266345"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH01050X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2004
/note="Mutations: 303:OK"
/codon_start=1
/product="calpastatin"
/protein_id="AAP88785.1"
/translation="MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVS
RSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESK
PDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTI
PPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEV
LKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDE
AKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSED
FDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDA
VEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDG
KPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPAS
TTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKL
GERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTT
ETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD"
BASE COUNT 720 a 446 c 471 g 367 t
ORIGIN
1 atgaatccca cagaaaccaa ggctgtaaaa acagaacctg agaagaagtc acagtcaacc
61 aagccaaaaa gcctacccaa gcaggcatca gatacaggaa gtaacgatgc tcacaataaa
121 aaagcagttt ccagatcagc tgaacagcag ccatcagaga aatcaacaga accaaagact
181 aaaccacaag acatgatttc tgctggtgga gagagtgttg ctggtatcac tgcaatatct
241 ggcaagccgg gtgacaagaa aaaagaaaag aaatcattaa ccccagctgt gccagttgaa
301 tccaaaccgg ataaaccatc gggaaagtca ggcatggatg ctgctttgga tgacttaata
361 gatactttag gaggacctga agaaactgaa gaagaaaata caacgtatac tggaccagaa
421 gtttcagatc caatgagttc cacctacata gaggaattgg gtaaaagaga agtcacaatt
481 cctccaaaat atagggaact attggctaaa aaggaaggga tcacagggcc tcctgcagac
541 tcttcgaaac ccatagggcc agatgatgct atagacgcct tgtcatctga cttcacctgt
601 gggtcgccta cagctgctgg aaagaaaact gaaaaagagg aatctacaga agttttaaaa
661 gctcagtcag cagggacagt cagaagtgct gctccacccc aagagaagaa aagaaaggtg
721 gagaaggata caatgagtga tcaagcactc gaggctctgt cggcttcact gggcacccgg
781 caagcagaac ctgagctcga cctccgctca attaaggaag tcgatgaggc aaaagctaaa
841 gaagaaaaac tagagaagtg tggtgaggat gatgaaacaa tcccatctga gtacagatta
901 aaaccagcca cggataaaga tggaaaacca ctattgccag agcctgaaga aaaacccaag
961 cctcggagtg aatcagaact cattgatgaa ctttcagaag attttgaccg gtctgaatgt
1021 aaagagaaac catctaagcc aactgaaaag acagaagaat ctaaggccgc tgctccagct
1081 cctgtgtcgg aggctgtgtg tcggacctcc atgtgtagta tacagtcagc accccctgag
1141 ccggctacct tgaagggcac agtgccagat gatgctgtag aagccttggc tgatagcctg
1201 gggaaaaagg aagcagatcc agaagatgga aaacctgtga tggataaagt caaggagaag
1261 gccaaagaag aagaccgtga aaagcttggt gaaaaagaag aaacaattcc tcctgattat
1321 agattagaag aggtcaagga taaagatgga aagccactcc tgccaaaaga gtctaaggaa
1381 cagcttccac ccatgagtga agacttcctt ctggatgctt tgtctgagga cttctctggt
1441 ccacaaaatg cttcatctct taaatttgaa gatgctaaac ttgctgctgc catctctgaa
1501 gtggtttccc aaaccccagc ttcaacgacc caagctggag ccccaccccg tgatacctcg
1561 cagagtgaca aagacctcga tgatgccttg gataaactct ctgacagtct aggacaaagg
1621 cagcctgacc cagatgagaa caaaccaatg gaagataaag taaaggaaaa agctaaagct
1681 gaacatagag acaagcttgg agaaagagat gacactatcc cacctgaata cagacatctc
1741 ctggatgata atggacagga caaaccagtg aagccaccta caaagaaatc agaggattca
1801 aagaaacctg cagatgacca agaccccatt gatgctctct caggagatct ggacagctgt
1861 ccctccacta cagaaacctc acagaacaca gcaaaggata agtgcaagaa ggctgcttcc
1921 agctccaaag cacctaagaa tggaggtaaa gcgaaggatt cagcaaagac aacagaggaa
1981 acttccaagc caaaagatga ctag
//