LOCUS BT007455 1968 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens forkhead box O1A (rhabdomyosarcoma) mRNA, complete
cds.
ACCESSION BT007455
VERSION BT007455.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1968)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1968)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1968
/db_xref="H-InvDB:HIT000100375"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00949X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1968
/codon_start=1
/product="forkhead box O1A (rhabdomyosarcoma)"
/protein_id="AAP36123.1"
/translation="MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAP
SGSAAANPDAAAGLPSASAAAVSADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAAA
TGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQHPPVPPAAAGPLAGQPRKSSSSRRNAW
GNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLS
LHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRSRAAKKKAS
LQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTE
QDDLGEGDVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQ
SSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPIQTLQDNKSSYG
GMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYG
SQASHNKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQVPL
PHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMD
GDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG"
BASE COUNT 450 a 609 c 533 g 376 t
ORIGIN
1 atggccgagg cgcctcaggt ggtggagatc gacccggact tcgagccgct gccccggccg
61 cgctcgtgca cctggccgct gcccaggccg gagtttagcc agtccaactc ggccacctcc
121 agcccggcgc cgtcgggcag cgcggctgcc aaccccgacg ccgcggcggg cctgccctcg
181 gcctcggctg ccgctgtcag cgccgacttc atgagcaacc tgagcttgct ggaggagagc
241 gaggacttcc cgcaggcgcc cggctccgtg gcggcggcgg tggcggcggc ggccgccgcg
301 gccgccaccg gggggctgtg cggggacttc cagggcccgg aggcgggctg cctgcaccca
361 gcgccaccgc agcccccgcc gcccgggccg ctgtcgcagc acccgccggt gccccccgcc
421 gccgctgggc cgctcgcggg gcagccgcgc aagagcagct cgtcccgccg caacgcgtgg
481 ggcaacctgt cctacgccga cctcatcacc aaggccatcg agagctcggc ggagaagcgg
541 ctcacgctgt cgcagatcta cgagtggatg gtcaagagcg tgccctactt caaggataag
601 ggtgacagca acagctcggc gggctggaag aattcaattc gtcataatct gtccctacac
661 agcaagttca ttcgtgtgca gaatgaagga actggaaaaa gttcttggtg gatgctcaat
721 ccagagggtg gcaagagcgg gaaatctcct aggagaagag ctgcatccat ggacaacaac
781 agtaaatttg ctaagagccg aagccgagct gccaagaaga aagcatctct ccagtctggc
841 caggagggtg ctggggacag ccctggatca cagttttcca aatggcctgc aagccctggc
901 tctcacagca atgatgactt tgataactgg agtacatttc gccctcgaac tagctcaaat
961 gctagtacta ttagtgggag actctcaccc attatgaccg aacaggatga tcttggagaa
1021 ggggatgtgc attctatggt gtacccgcca tctgccgcaa agatggcctc tactttaccc
1081 agtctgtctg agataagcaa tcccgaaaac atggaaaatc ttttggataa tctcaacctt
1141 ctctcatcac caacatcatt aactgtttcg acccagtcct cacctggcac catgatgcag
1201 cagacgccgt gctactcgtt tgcgccacca aacaccagtt tgaattcacc cagcccaaac
1261 taccaaaaat atacatatgg ccaatccagc atgagccctt tgccccagat gcctatacaa
1321 acacttcagg acaataagtc gagttatgga ggtatgagtc agtataactg tgcgcctgga
1381 ctcttgaagg agttgctgac ttctgactct cctccccata atgacattat gacaccagtt
1441 gatcctgggg tagcccagcc caacagccgg gttctgggcc agaacgtcat gatgggccct
1501 aattcggtca tgtcaaccta tggcagccag gcatctcata acaaaatgat gaatcccagc
1561 tcccataccc accctggaca tgctcagcag acatctgcag ttaacgggcg tcccctgccc
1621 cacacggtaa gcaccatgcc ccacacctcg ggtatgaacc gcctgaccca agtgaagaca
1681 cctgtacaag tgcctctgcc ccaccccatg cagatgagtg ccctgggggg ctactcctcc
1741 gtgagcagct gcaatggcta tggcagaatg ggccttctcc accaggagaa gctcccaagt
1801 gacttggatg gcatgttcat tgagcgctta gactgtgaca tggaatccat cattcggaat
1861 gacctcatgg atggagatac attggatttt aactttgaca atgtgttgcc caaccaaagc
1921 ttcccacaca gtgtcaagac aacgacacat agctgggtgt caggctag
//