LOCUS BT007419 2601 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens peptidylglycine alpha-amidating monooxygenase mRNA,
complete cds.
ACCESSION BT007419
VERSION BT007419.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2601)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2601)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2601
/db_xref="H-InvDB:HIT000100339"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00961X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2601
/codon_start=1
/product="peptidylglycine alpha-amidating monooxygenase"
/protein_id="AAP36087.1"
/translation="MAGRVPSLLVLLVFPSSCLAFRSPLSVFKRFKETTRPFSNECLG
TTRPVVPIDSSDFALDIRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVH
HMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSK
YFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNS
DISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPV
DVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVA
PDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQDFHME
EALDWPGVYLLPGQVSGVALDPKNNLVIFHRGDHVWDGNSFDSKFVYQQIGLGPIEED
TILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALHQVFKLDPNNKEGPVL
ILGRSMQPGSDQNHFCQPTDVAVDPGTGAIYVSDGYCNSRIVQFSPSGKFITQWGEES
SGSSPLPGQFTVPHSLALVPLLGQLCVADRENGRIQCFKTDTKEFVREIKHSSFGRNV
FAISYIPGLLFAVNGKPHFGDQEPVQGFVMNFSNGEIIDIFKPVRKHFDMPHDIVASE
DGTVYIGDAHTNTVWKFTLTEKLEHRSVKKAGIEVQEIKEAEAVVETKMENKPTSSEL
QKMQEKQKLIKEPGSGVPVVLITTLLVIPVVVLLAIAIFIRWKKSRAFGDSEHKLETS
SGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRLSTEGSDQEKEDDGSESEEEYSA
PLPALAPSSS"
BASE COUNT 740 a 543 c 628 g 690 t
ORIGIN
1 atggctggcc gcgtccctag cctgctagtt ctccttgttt ttccaagcag ctgtttggct
61 ttccgaagcc cactttctgt ctttaagagg tttaaagaaa ctaccagacc attttccaat
121 gaatgtcttg gtaccaccag acccgtagtt cctattgatt catcagattt tgcattggat
181 attcgcatgc ctggggttac acctaaacag tccgatacat acttctgcat gtctatgcga
241 ataccagtgg atgaggaagc cttcgtgatt gacttcaagc ctcgagccag catggatact
301 gtccatcaca tgttactttt tggatgcaat atgccttcat ccactggaag ttactggttt
361 tgtgatgaag gaacctgtac agataaagcc aatattctgt atgcctgggc gagaaatgct
421 ccccctaccc ggctccccaa aggtgttgga ttcagagttg gaggagagac tggaagtaaa
481 tactttgtac tacaggtaca ctatggggat attagtgctt ttagagataa taacaaggac
541 tgttctggtg tgtccttaca cctcacacgt ctgccacagc ctttaattgc tggcatgtac
601 cttatgatgt ctgttgacac tgttatccca gcaggagaaa aagtggtgaa ttctgacatt
661 tcatgccatt ataaaaatta tccaatgcat gtctttgcct atagagttca cactcaccat
721 ttaggtaagg tagtaagtgg atacagagta agaaatggac agtggacact gattggacgg
781 cagagccctc agctgccaca ggctttctac cctgtggggc atccagttga tgtaagtttt
841 ggtgacctac tggctgcaag atgtgtattc actggtgaag gaaggacaga agccacacac
901 attggtggca cgtctagtga tgaaatgtgc aacttataca ttatgtatta catggaagcc
961 aagcatgcag tttctttcat gacctgtacc cagaatgtag ctccagatat gttcagaacc
1021 ataccaccag aggccaacat tccaattccc gtgaagtctg atatggttat gatgcatgaa
1081 catcataaag aaacagaata taaagataag attcctttac tacagcagcc aaaacgagaa
1141 gaagaagaag tgttagacca ggatttccac atggaagagg cactggattg gcctggagta
1201 tacttgttac caggccaggt ttctggggtg gctctagacc ctaagaataa cctggtgatt
1261 ttccacagag gtgaccatgt ctgggatgga aactcgtttg acagcaagtt tgtttaccag
1321 caaataggac tcggaccaat tgaagaagac actattcttg tcatagatcc aaataatgct
1381 gcagtactcc agtccagtgg aaaaaatctg ttttacttgc cacatggctt gagtatagat
1441 aaagatggga attattgggt cacagacgtg gctctccatc aggtgttcaa actggatcca
1501 aacaataaag aaggccctgt attaatcctg ggaaggagca tgcaaccagg cagtgaccag
1561 aatcacttct gtcaacccac tgatgtggct gtggatccag gcactggagc catttatgta
1621 tcagatggtt actgcaacag caggattgtg cagttttcac caagtggaaa gttcatcaca
1681 cagtggggag aagagtcttc agggagcagt cctctgccag gccagttcac tgttcctcac
1741 agcttggctc ttgtgcctct tttgggccaa ttatgtgtgg cagaccggga aaatggtcgg
1801 atccagtgtt ttaaaactga caccaaagaa tttgtgagag agattaagca ttcatcattt
1861 ggaagaaatg tatttgcaat ttcatatata ccaggcttgc tctttgcagt gaatgggaag
1921 cctcattttg gggaccaaga acctgtacaa ggatttgtga tgaacttttc caatggggaa
1981 attatcgaca tcttcaagcc agtgcgcaag cactttgata tgcctcatga tattgttgca
2041 tctgaagatg ggactgtgta cattggagat gctcatacca acaccgtgtg gaagttcacc
2101 ttgactgaga aattggaaca tcgatcagtt aaaaaggctg gcattgaggt ccaggaaatc
2161 aaagaagccg aggcagttgt tgaaaccaaa atggagaaca aacccacctc ctcagaattg
2221 cagaagatgc aagagaaaca gaaactgatc aaagagccag gctcgggagt gcctgttgtt
2281 ctcattacaa cccttctggt tattccggtg gttgtcctgc tggccattgc catatttatt
2341 cggtggaaaa aatcaagggc ctttggagat tctgaacaca aactcgagac gagttcagga
2401 agagtactgg gaagatttag aggaaaggga agtggaggct taaaccttgg taatttcttt
2461 gcaagccgta agggctacag tcgaaaaggg tttgaccggc ttagcactga gggcagtgac
2521 caagagaaag aggatgatgg aagtgaatca gaagaggagt attcagcacc tctgcctgcg
2581 ctcgcacctt cctcctccta g
//