LOCUS BT007394 1983 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens arginyl-tRNA synthetase mRNA, complete cds.
ACCESSION BT007394
VERSION BT007394.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1983)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1983)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1983
/db_xref="H-InvDB:HIT000100314"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00984X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1983
/note="Mutations: 1674:OK"
/codon_start=1
/product="arginyl-tRNA synthetase"
/protein_id="AAP36058.1"
/translation="MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQL
QEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLV
TPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPG
FINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVGHLRSTIIG
ESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKES
KKRFDTEEEFKKRAYQCVVLLQGKNPDITKAWKLICDVSRQELNKIYDALDVSLIERG
ESFYQDRMNDIVKEFEDRGFVQVDDGRKIVFVPGCSIPLTIVKSDGGYTYDTSDLAAI
KQRLFEEKADMIIYVVDNGQSVHFQTIFAAAQMIGWYDPKVTRVFHAGFGVVLGEDKK
KFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEELNAAQTSVAYGCIKYADL
SHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANIDEEMLQKAARETKILL
DHEKEWKLGRCILRFPEILQKILDDLFLHTLCDYIYELATAFTEFYDSCYCVEKDRQT
GKILKVNMWRMLLCEAVAAVMAKGFDILGIKPVQRM"
BASE COUNT 626 a 351 c 463 g 543 t
ORIGIN
1 atggacgtac tggtgtctga gtgctccgcg cggctgctgc agcaggaaga agagattaaa
61 tctctgactg ctgaaattga ccggttgaaa aactgtggct gtttaggagc ttctccaaat
121 ttggagcagt tacaagaaga aaatttaaaa ttaaagtatc gactgaatat tcttcgaaag
181 agtcttcagg cagaaaggaa caaaccaact aaaaatatga ttaacattat tagccgccta
241 caagaggtct ttggtcatgc aattaaggct gcatatccag atttggaaaa tcctcctctg
301 ctagtgacac caagtcagca ggccaagttt ggggactatc agtgtaatag tgctatgggt
361 atttctcaga tgctcaaaac caaggaacag aaagttaatc caagagaaat tgctgaaaac
421 attaccaaac acctcccaga caatgaatgt attgaaaaag ttgaaattgc tggtcctggt
481 tttattaatg tccacttaag aaaggatttt gtatcagaac aattgaccag tcttctagtg
541 aatggagttc aactacctgc tctgggagag aataaaaagg ttatagttga cttttcctcc
601 cctaatatag ctaaagagat gcatgtaggc cacctgaggt caactatcat aggagagagt
661 ataagccgcc tctttgaatt tgcagggtat gacgtgctca ggttaaatca tgtaggagac
721 tgggggaccc agtttggcat gctcatcgct cacctgcaag acaaatttcc agattatcta
781 acagtttcac ctcctattgg ggatcttcag gtcttttata aggaatctaa gaagaggttt
841 gatactgagg aggaatttaa gaagcgagca tatcagtgtg tagttctgct ccagggtaaa
901 aacccagata ttacaaaagc ttggaagctt atctgtgatg tctcccgcca agagttaaat
961 aaaatctatg atgcattgga cgtctcttta atagagagag gggaatcctt ctatcaagat
1021 aggatgaatg atattgtaaa ggaatttgaa gatagaggat ttgtgcaggt ggatgatggc
1081 agaaagattg tatttgtccc agggtgttcc ataccattaa ccatagtaaa atcagatgga
1141 ggttatacct atgatacatc tgacctggct gctattaaac aaagactatt tgaggaaaaa
1201 gcagatatga ttatctatgt tgtggacaat ggacaatctg tgcacttcca gacaatattt
1261 gctgctgctc aaatgattgg ttggtatgac cctaaagtaa ctcgagtctt ccatgctgga
1321 tttggtgtgg tgctagggga agacaagaaa aagtttaaaa cacgttcggg tgaaacagtg
1381 cgcctcatgg atcttctggg agaaggacta aaacgatcca tggacaagtt gaaggaaaaa
1441 gaaagagaca aggtcttaac tgcagaggaa ttgaatgctg ctcagacatc cgttgcatat
1501 ggctgcatca aatatgctga cctttcccat aaccggttga atgactacat cttctccttt
1561 gacaaaatgc tagatgacag aggaaataca gctgcttact tgttgtatgc cttcactaga
1621 atcaggtcta ttgcacgtct ggccaatatt gatgaagaaa tgctccaaaa agcagctcga
1681 gaaaccaaga ttcttttgga tcatgagaag gaatggaaac taggccggtg cattttacgg
1741 ttccctgaga ttctgcaaaa gattttagat gacttatttc tccacactct ctgtgattat
1801 atatatgagc tggcaactgc tttcacagag ttctatgata gctgctactg tgtggagaaa
1861 gatagacaga ctggaaaaat attgaaggtg aacatgtggc gtatgctgct atgtgaagca
1921 gtagctgctg tcatggccaa ggggtttgat atcctgggaa taaaacctgt ccaaaggatg
1981 tag
//