LOCUS BT007318 1215 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens diaphanous homolog 1 (Drosophila) mRNA, complete cds.
ACCESSION BT007318
VERSION BT007318.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1215)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1215)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1215
/db_xref="H-InvDB:HIT000100238"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00890X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1215
/codon_start=1
/product="diaphanous homolog 1 (Drosophila)"
/protein_id="AAP35982.1"
/translation="MPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKD
EYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKS
ESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCE
NDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKF
VEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNM
FLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSL
LEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTI
LEEAKELVGRAS"
BASE COUNT 354 a 254 c 328 g 279 t
ORIGIN
1 atgccctatc aagagattaa gaatgtcatc ctggaggtga atgaggctgt tctgactgag
61 tctatgatcc agaacctcat taagcaaatg ccagagccag agcagttaaa aatgctttct
121 gaactgaagg atgaatatga tgacctggct gagtcagagc agtttggcgt ggtgatgggc
181 actgtgcccc gactgcggcc tcgcctcaat gccattctct tcaagctaca attcagcgag
241 caagtggaga atatcaagcc agagattgtg tctgtcactg ctgcatgtga ggagttacgt
301 aagagtgaga gcttttccaa tctcctagag attaccttgc ttgttggaaa ttacatgaat
361 gctggctcca gaaatgctgg tgcttttggc ttcaatatca gcttcctctg taagcttcga
421 gacaccaagt ccacagatca gaagatgacg ttgttacact tcttggctga gttgtgtgag
481 aatgactatc ccgatgtcct caagtttcca gacgagcttg cccatgtgga gaaagccagc
541 cgagtttctg ctgaaaactt gcaaaagaac ctagatcaga tgaagaaaca aatttctgat
601 gtggaacgtg atgttcagaa tttcccagct gccacagatg aaaaagacaa gtttgttgaa
661 aaaatgacca gctttgtgaa ggatgcacag gaacagtata acaagctgcg gatgatgcat
721 tctaacatgg agaccctcta taaggagctg ggcgagtact tcctctttga ccccaagaag
781 ttgtctgttg aagaattttt catggatctt cacaattttc ggaatatgtt tttgcaagca
841 gtcaaggaga accagaagcg gcgggagaca gaagaaaaga tgaggcgagc aaaactagcc
901 aaggagaagg cagagaagga gcggctagag aagcagcaga agagagagca actcatagac
961 atgaatgcag agggcgatga gacaggtgtg atggacagtc ttctagaagc cctgcagtca
1021 ggggcagcat tccgacggaa gagagggccc cgtcaagcca acaggaaggc cgggtgtgca
1081 gtcacatctc tgctagcttc ggagctgacc aaggatgatg ccatggctgc tgttcctgcc
1141 aaggtgtcca agaacagtga gacattcccc acaatccttg aggaagccaa ggagttggtt
1201 ggccgtgcaa gctag
//