LOCUS BT007172 1752 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens hypothetical protein from BCRA2 region mRNA, complete
cds.
ACCESSION BT007172
VERSION BT007172.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1752)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1752)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1752
/db_xref="H-InvDB:HIT000100092"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00048X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1752
/codon_start=1
/product="hypothetical protein from BCRA2 region"
/protein_id="AAP35836.1"
/translation="MSYGEIEGKFLGPREEVTSEPRCKKLKSTTESYVFHNHSNADFH
RIQEKTGNDWVPVTIIDVRGHSYLQENKIKTTDLHRPLHDEMPGNRPDVIESIDSQVL
QEARPPLVSADDEIYSTSKAFIGPIYKPPEKKKRNEGRNEAHVLNGINDRGGQKEKQK
FNSEKSEIDNELFQFYKEIEELEKEKDGFENSCKESEPSQEQFVPFYEGHNNGLLKPD
EEKKDLSNKAMPSHCDYQQNLGNEPDKYPCNGQVIPTFCDTSFTSFRPEWQSVYPFIV
PYGPPLPSLNYHLNIQRFSGPPNPPSNIFQAQDDSQIQNGYYVNNCHVNWNCMTFDQN
NEYTDCSENRSSVHPSGNGCSMQDRYVSNGFCEVRERCWKDHCMDKHNGTDRFVNQQF
QEEKLNKLQKLLILLRGLPGSGKTTLSRILLGQNRDGIVFSTDDYFHHQDGYRYNVNQ
LGDAHDWNQNRAKQAIDQGRSPVIIDNTNIQAWEMKPYVEVAIGKGYRVEFHEPETWW
KFDPEELEKRNKHGVSRKKIAQMLDRYEYQMSISIVMNSVEPSHKSTQRPPPPQGRQR
WGGSLGSHNRVCVTNNH"
BASE COUNT 611 a 292 c 376 g 473 t
ORIGIN
1 atgtcttatg gtgaaattga aggtaaattc ttgggaccta gagaagaagt aacgagtgag
61 ccacgctgta aaaaattgaa gtcaaccaca gagtcgtatg tttttcacaa tcatagtaat
121 gctgattttc acagaatcca agagaaaact ggaaatgatt gggtccctgt gaccatcatt
181 gatgtcagag gacatagtta tttgcaggag aacaaaatca aaactacaga tttgcataga
241 cctttgcatg atgagatgcc tggtaataga ccagatgtta ttgaatccat tgattcacag
301 gttttacagg aagcacgtcc tccattagta tccgcagacg atgagatata tagcacaagt
361 aaagcattta taggacccat ttacaaaccc cctgagaaaa agaaacgtaa tgaagggagg
421 aatgaggcac atgttctaaa tggtataaat gacagaggag gacaaaaaga gaaacagaaa
481 tttaactctg aaaaatcaga gattgacaat gaattattcc agttttacaa agaaattgaa
541 gagcttgaaa aggaaaaaga tggttttgag aacagttgta aagaatctga accttctcag
601 gaacaatttg ttccatttta tgagggtcat aataatggtc tcttaaaacc tgatgaagaa
661 aagaaagatc ttagtaataa agctatgcca tcacattgtg attatcagca gaacttgggg
721 aatgagccag acaaatatcc ctgtaatgga caagtaatac ctacattttg tgacacttca
781 tttacttctt tcaggcctga atggcagtca gtatatcctt ttatagtgcc ctatggtccc
841 cctcttccca gtttgaacta tcatttaaac attcagagat tcagtggtcc accaaatcca
901 ccatcaaata ttttccaagc ccaagatgac tctcagatac aaaatggata ttatgtaaat
961 aattgtcatg ttaactggaa ttgcatgact tttgatcaga acaatgaata tactgactgt
1021 agtgagaata ggagtagtgt tcatccctct ggaaatggct gcagtatgca agatcgatat
1081 gtgagtaatg gtttctgtga agtcagagaa agatgctgga aagatcattg tatggacaag
1141 cataatggaa cagacaggtt tgtgaaccag cagtttcaag aggaaaagtt aaataaattg
1201 cagaagttac ttattctttt aagaggtctg cctggttctg ggaaaacaac attgtctcga
1261 attctgcttg gtcagaatcg tgatggcatt gtgttcagca ctgatgacta ttttcaccat
1321 caagatgggt acaggtataa tgttaatcaa cttggtgatg cccatgactg gaaccagaac
1381 agagcaaaac aagctatcga tcagggaaga tctccagtta taatagataa cactaatata
1441 caagcttggg aaatgaagcc atatgtggaa gtggccatag gaaaaggata cagagtagag
1501 tttcatgaac ctgaaacttg gtggaaattt gatcctgaag aattagaaaa gaggaataaa
1561 catggtgtgt ctcgaaagaa gattgctcag atgttggatc gttatgaata tcaaatgtcc
1621 atttctattg taatgaattc agtggaacca tcacacaaaa gcacacaaag acctcctcct
1681 ccacagggga gacagaggtg gggaggctct cttggctcac ataatcgtgt ctgtgtcaca
1741 aataatcatt ag
//