LOCUS BT007163 1305 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens enolase 1, (alpha) mRNA, complete cds.
ACCESSION BT007163
VERSION BT007163.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1305)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1305)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1305
/db_xref="H-InvDB:HIT000100083"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00764X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1305
/codon_start=1
/product="enolase 1, (alpha)"
/protein_id="AAP35827.1"
/translation="MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIY
EALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTEN
KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAG
NKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNIL
ENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQL
ADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNE
KSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQ
IKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK"
BASE COUNT 323 a 325 c 371 g 286 t
ORIGIN
1 atgtctattc tcaagatcca tgccagggag atctttgact ctcgcgggaa tcccactgtt
61 gaggttgatc tcttcacctc aaaaggtctc ttcagagctg ctgtgcccag tggtgcttca
121 actggtatct atgaggccct agagctccgg gacaatgata agactcgcta tatggggaag
181 ggtgtctcaa aggctgttga gcacatcaat aaaactattg cgcctgccct ggttagcaag
241 aaactgaacg tcacagaaca agagaagatt gacaaactga tgatcgagat ggatggaaca
301 gaaaataaat ctaagtttgg tgcgaacgcc attctggggg tgtcccttgc cgtctgcaaa
361 gctggtgccg ttgagaaggg ggtccccctg taccgccaca tcgctgactt ggctggcaac
421 tctgaagtca tcctgccagt cccggcgttc aatgtcatca atggcggttc tcatgctggc
481 aacaagctgg ccatgcagga gttcatgatc ctcccagtcg gtgcagcaaa cttcagggaa
541 gccatgcgca ttggagcaga ggtttaccac aacctgaaga atgtcatcaa ggagaaatat
601 gggaaagatg ccaccaatgt gggggatgaa ggcgggtttg ctcccaacat cctggagaat
661 aaagaaggcc tggagctgct gaagactgct attgggaaag ctggctacac tgataaggtg
721 gtcatcggca tggacgtagc ggcctccgag ttcttcaggt ctgggaagta tgacctggac
781 ttcaagtctc ccgatgaccc cagcaggtac atctcgcctg accagctggc tgacctgtac
841 aagtccttca tcaaggacta cccagtggtg tctatcgaag atccctttga ccaggatgac
901 tggggagctt ggcagaagtt cacagccagt gcaggaatcc aggtagtggg ggatgatctc
961 acagtgacca acccaaagag gatcgccaag gccgtgaacg agaagtcctg caactgcctc
1021 ctgctcaaag tcaaccagat tggctccgtg accgagtctc ttcaggcgtg caagctggcc
1081 caggccaatg gttggggcgt catggtgtct catcgttcgg gggagactga agataccttc
1141 atcgctgacc tggttgtggg gctgtgcact gggcagatca agactggtgc cccttgccga
1201 tctgagcgct tggccaagta caaccagctc ctcagaattg aagaggagct gggcagcaag
1261 gctaagtttg ccggcaggaa cttcagaaac cccttggcca agtag
//