LOCUS       BT007153                 567 bp    mRNA    linear   HUM 13-MAY-2003
DEFINITION  Homo sapiens v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
            mRNA, complete cds.
ACCESSION   BT007153
VERSION     BT007153.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 567)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
            Circle, Palo Alto, CA 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..567
                     /db_xref="H-InvDB:HIT000100073"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH00755X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..567
                     /codon_start=1
                     /product="v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene
                     homolog"
                     /protein_id="AAP35817.1"
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV
                     VIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKR
                     VKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLV
                     REIRKHKEKMSKDGKKKKKKSKTKCVIM"
BASE COUNT          208 a           80 c          134 g          145 t
ORIGIN      
        1 atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgacg
       61 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac
      121 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt
      181 catgaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt
      241 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt
      301 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg
      361 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct
      421 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt
      481 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag
      541 tcaaagacaa agtgtgtaat tatgtag
//