LOCUS BT007147 2034 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens galactosidase, beta 1 mRNA, complete cds.
ACCESSION BT007147
VERSION BT007147.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2034)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2034)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2034
/db_xref="H-InvDB:HIT000100067"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00254X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2034
/codon_start=1
/product="galactosidase, beta 1"
/protein_id="AAP35811.1"
/translation="MPGFLVRILLLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKD
GQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDH
DVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDK
WLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLF
TTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDH
WGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYD
APLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDIL
CPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGI
PQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTD
WTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLP
QDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWA
PCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV"
BASE COUNT 475 a 552 c 511 g 496 t
ORIGIN
1 atgccggggt tcctggttcg catcctcctt ctgctgctgg ttctgctgct tctgggccct
61 acgcgcggct tgcgcaatgc cacccagagg atgtttgaaa ttgactatag ccgggactcc
121 ttcctcaagg atggccagcc atttcgctac atctcaggaa gcattcacta ctcccgtgtg
181 ccccgcttct actggaagga ccggctgctg aagatgaaga tggctgggct gaacgccatc
241 cagacgtatg tgccctggaa ctttcatgag ccctggccag gacagtacca gttttctgag
301 gaccatgatg tggaatattt tcttcggctg gctcatgagc tgggactgct ggttatcctg
361 aggcccgggc cctacatctg tgcagagtgg gaaatgggag gattacctgc ttggctgcta
421 gagaaagagt ctattcttct ccgctcctcc gacccagatt acctggcagc tgtggacaag
481 tggttgggag tccttctgcc caagatgaag cctctcctct atcagaatgg agggccagtt
541 ataacagtgc aggttgaaaa tgaatatggc agctactttg cctgtgattt tgactacctg
601 cgcttcctgc agaagcgctt tcgccaccat ctgggggatg atgtggttct gtttaccact
661 gatggagcac ataaaacatt cctgaaatgt ggggccctgc agggcctcta caccacggtg
721 gactttggaa caggcagcaa catcacagat gctttcctaa gccagaggaa gtgtgagccc
781 aaaggaccct tgatcaattc tgaattctat actggctggc tagatcactg gggccaacct
841 cactccacaa tcaagaccga agcagtggct tcctccctct atgatatact tgcccgtggg
901 gcgagtgtga acttgtacat gtttataggt gggaccaatt ttgcctattg gaatggggcc
961 aactcaccct atgcagcaca gcccaccagc tacgactatg atgccccact gagtgaggct
1021 ggggacctca ctgagaagta ttttgctctg cgaaacatca tccagaagtt tgaaaaagta
1081 ccagaaggtc ctatccctcc atctacacca aagtttgcat atggaaaggt cactttggaa
1141 aagttaaaga cagtgggagc agctctggac attctgtgtc cctctgggcc catcaaaagc
1201 ctttatccct tgacatttat ccaggtgaaa cagcattatg ggtttgtgct gtaccggaca
1261 acacttcctc aagattgcag caacccagca cctctctctt cacccctcaa tggagtccac
1321 gatcgagcat atgttgctgt ggatgggatc ccccagggag tccttgagcg aaacaatgtg
1381 atcactctga acataacagg gaaagctgga gccactctgg accttctggt agagaacatg
1441 ggacgtgtga actatggtgc atatatcaac gattttaagg gtttggtttc taacctgact
1501 ctcagttcca atatcctcac ggactggacg atctttccac tggacactga ggatgcagtg
1561 cgcagccacc tggggggctg gggacaccgt gacagtggcc accatgatga agcctgggcc
1621 cacaactcat ccaactacac gctcccggcc ttttatatgg ggaacttctc cattcccagt
1681 gggatcccag acttgcccca ggacaccttt atccagtttc ctggatggac caagggccag
1741 gtctggatta atggctttaa ccttggccgc tattggccag cccggggccc tcagttgacc
1801 ttgtttgtgc cccagcacat cctgatgacc tcggccccaa acaccatcac cgtgctggaa
1861 ctggagtggg caccctgcag cagtgatgat ccagaactat gtgctgtgac gttcgtggac
1921 aggccagtta ttggctcatc tgtgacctac gatcatccct ccaaacctgt tgaaaaaaga
1981 ctcatgcccc cacccccgca aaaaaacaaa gattcatggc tggaccatgt atag
//