LOCUS BT007145 1272 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens isovaleryl Coenzyme A dehydrogenase mRNA, complete
cds.
ACCESSION BT007145
VERSION BT007145.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1272)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1272)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1272
/db_xref="H-InvDB:HIT000100065"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00054X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1272
/codon_start=1
/product="isovaleryl Coenzyme A dehydrogenase"
/protein_id="AAP35809.1"
/translation="MATATRLLGCRVASWRLRPPLAGFVSQRAHSLLPVDDAINGLSE
EQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSG
LGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIG
ALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPA
SRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVL
MSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLM
ACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGR
FLRDAKLYEIGAGTSEVRRLVIGRAFNADFH"
BASE COUNT 272 a 326 c 395 g 279 t
ORIGIN
1 atggcgactg cgactcggct gctggggtgt cgtgtggcga gctggaggct gcggccgccg
61 cttgccggct tcgtttccca gcgggcccac tcgcttttgc ccgtggacga tgcaatcaat
121 gggctaagcg aggagcagag gcagcttcgt cagaccatgg ctaagttcct tcaggagcac
181 ctggccccca aggcccagga gatcgatcgc agcaatgagt tcaagaacct gcgagaattt
241 tggaagcagc tggggaacct gggcgtattg ggcatcacag cccctgttca gtatggcggc
301 tccggcctgg gctacctgga gcatgtgctg gtgatggagg agatatcccg agcttccgga
361 gcagtggggc tcagttacgg tgcccactcc aacctctgca tcaaccagct tgtacgcaat
421 gggaatgagg cccagaaaga gaagtatctc ccgaagctga tcagtggtga gtacatcgga
481 gccctggcca tgagtgagcc caatgcaggc tctgatgttg tctctatgaa gctcaaagcg
541 gaaaagaaag gaaatcacta catcctgaat ggcaacaagt tctggatcac taatggccct
601 gatgctgacg tcctgattgt ctatgccaag acagatctgg ctgctgtgcc agcttctcgg
661 ggcatcacag ccttcattgt ggagaagggt atgcctggct ttagcacctc taagaagctg
721 gacaagctgg ggatgagggg ctctaacacc tgtgagctaa tctttgaaga ctgcaagatt
781 cctgctgcca acatcctggg ccatgagaat aagggtgtct acgtgctgat gagtgggctg
841 gacctggagc ggctggtgct ggccgggggg cctcttgggc tcatgcaagc ggtcctggac
901 cacaccattc cctacctgca cgtgagggaa gcctttggcc agaagatcgg ccacttccag
961 ttgatgcagg ggaagatggc tgacatgtac acccgcctca tggcgtgtcg gcagtatgtc
1021 tacaatgtcg ccaaggcctg cgatgagggc cattgcactg ctaaggactg tgcaggtgtg
1081 attctttact cagctgagtg tgccacacag gtagccctgg acggcattca gtgttttggt
1141 ggcaatggct acatcaatga ctttcccatg ggccgctttc ttcgagatgc caagctgtat
1201 gagatagggg ctgggaccag cgaggtgagg cggctggtca tcggcagagc cttcaatgca
1261 gactttcact ag
//