LOCUS BT007120 489 bp mRNA linear HUM 13-MAY-2003 DEFINITION Homo sapiens basic transcription factor 3 mRNA, complete cds. ACCESSION BT007120 VERSION BT007120.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 489) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 489) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow Circle, Palo Alto, CA 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..489 /db_xref="H-InvDB:HIT000100040" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH00725X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..489 /codon_start=1 /product="basic transcription factor 3" /protein_id="AAP35784.1" /translation="MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKL QFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEM LPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNE AN" BASE COUNT 169 a 99 c 118 g 103 t ORIGIN 1 atgaaagaaa caatcatgaa ccaggaaaaa ctcgccaaac tgcaggcaca agtgcgcatt 61 ggtgggaaag gaactgctcg cagaaagaag aaggtggttc atagaacagc cacagcagat 121 gacaaaaaac ttcagttctc cttaaagaag ttaggggtaa acaatatctc tggtattgaa 181 gaggtgaata tgtttacaaa ccaaggaaca gtgatccact ttaacaaccc taaagttcag 241 gcatctctgg cagcgaacac tttcaccatt acaggccatg ctgagacaaa gcagctgaca 301 gaaatgctac ccagcatctt aaaccagctt ggtgcggata gtctgactag tttaaggaga 361 ctggccgaag ctctgcccaa acaatctgtg gatggaaaag caccacttgc tactggagag 421 gatgatgatg atgaagttcc agatcttgtg gagaattttg atgaggcttc caagaatgag 481 gcaaactag //