LOCUS BT007119 1950 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens RecQ protein-like (DNA helicase Q1-like) mRNA,
complete cds.
ACCESSION BT007119
VERSION BT007119.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1950)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1950)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1950
/db_xref="H-InvDB:HIT000100039"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00256X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1950
/codon_start=1
/product="RecQ protein-like (DNA helicase Q1-like)"
/protein_id="AAP35783.1"
/translation="MASVSALTEELDSITSELHAVEIQIQELTERQQELIQKKKVLTK
KIKQCLEDSDAGASNEYDSSPAAWNKEDFPWSGKVKDILQNVFKLEKFRPLQLETINV
TMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATM
LNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAV
DEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFT
FTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSL
QNLGIHAGAYHANLEPEDKTTVHRKWSANEIQVVVATVAFGMGIDKPDVRFVIHHSMS
KSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVGQQKLYEMVSYCQNI
SKCRRVLMAQHFDEVWNSEACNKMCDNCCKDSAFERKNITEYCRDLIKILKQAEELNE
KLTPLKLIDSWMGKGAAKLRVAGVVAPTLPREDLEKIIAHFLIQQYLKEDYSFTAYAT
ISYLKIGPKANLLNNEAHAITMQVTKSTQNSFRAESSQTCHSEQGDKKMEKKNSGNFQ
KKAANMLQQSGSKNTGAKKRKIDDA"
BASE COUNT 661 a 323 c 435 g 531 t
ORIGIN
1 atggcgtccg tttcagctct aactgaggaa ctggattcta taaccagtga gctacatgca
61 gtagaaattc aaattcaaga acttacggaa aggcaacaag agcttattca gaaaaaaaaa
121 gtcctgacaa agaaaataaa gcagtgttta gaggattctg atgccggggc aagcaatgaa
181 tatgattctt cacctgccgc ttggaataaa gaagattttc catggtctgg taaagttaaa
241 gatattctgc aaaatgtctt taaactggaa aagttcagac cacttcagct tgaaactatt
301 aacgtaacaa tggctggaaa ggaggtattt cttgttatgc ctacaggagg tggaaagagc
361 ttatgttacc agttaccagc attatgttca gatggtttta cactcgtcat ttgcccattg
421 atctctctta tggaagacca attaatggtt ttaaaacaat taggaatttc agcaaccatg
481 ttaaatgctt ctagttctaa ggagcatgtt aaatgggttc atgctgaaat ggtaaataaa
541 aactccgagt taaagctgat ttatgtgact ccagagaaaa ttgcaaaaag caaaatgttt
601 atgtcaagac tagagaaagc ctatgaagca aggagattta ctcgaattgc tgtggatgaa
661 gttcactgct gtagtcagtg gggacatgat ttcagacctg attataaggc acttggtatc
721 ttaaagcggc agttccctaa cgcatcacta attgggctga ctgcaactgc aacaaatcac
781 gttttgacgg atgctcagaa aattttgtgc attgaaaagt gttttacttt tacagcttct
841 tttaataggc caaatctata ttatgaggtt cggcagaagc cctcaaacac tgaagatttt
901 attgaggata ttgtaaagct cattaatggg agatacaaag ggcaatcagg aatcatatat
961 tgtttttctc agaaagactc tgaacaagtt acggttagtt tgcagaatct gggaattcat
1021 gcaggtgctt accatgccaa tttggagcca gaagataaga ccacagttca tagaaaatgg
1081 tcagccaatg aaattcaggt agtagtggca actgttgcat ttggtatggg aattgataag
1141 ccagatgtga ggtttgttat ccatcattca atgagtaaat ccatggaaaa ttattaccaa
1201 gagagtggac gtgcaggtcg agatgacatg aaagcagact gtattttgta ctacggcttt
1261 ggagatatat tcagaataag ttcaatggtg gtgatggaaa atgtgggaca gcagaagctt
1321 tatgagatgg tatcatactg tcaaaacata agcaaatgtc gtcgtgtgtt gatggctcaa
1381 cattttgatg aagtatggaa ctcagaagca tgtaacaaaa tgtgcgataa ctgctgtaaa
1441 gacagtgcat ttgaaagaaa gaacataaca gagtactgca gagatctaat caagatcctg
1501 aagcaggcag aggaactgaa tgaaaaactc actccattga aactgattga ttcttggatg
1561 ggaaagggtg cagcaaaact gagagtagca ggtgttgtgg ctcccacact tcctcgtgaa
1621 gatctggaga agattattgc acactttcta atacagcagt atcttaaaga agactacagt
1681 tttacagctt atgctaccat ttcgtatttg aaaataggac ctaaagctaa ccttctgaac
1741 aatgaggcac atgctattac tatgcaagtg acaaagtcca cgcagaactc tttcagggct
1801 gaatcgtctc aaacttgtca ttctgaacaa ggtgataaaa agatggagaa aaaaaattca
1861 ggcaacttcc agaagaaggc tgcaaacatg cttcagcagt ctggttctaa gaatacagga
1921 gctaagaaaa gaaaaatcga tgatgcctag
//