LOCUS       BT007087                 543 bp    mRNA    linear   HUM 13-MAY-2003
DEFINITION  Homo sapiens ADP-ribosylation factor 5 mRNA, complete cds.
ACCESSION   BT007087
VERSION     BT007087.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 543)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
            Circle, Palo Alto, CA 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..543
                     /db_xref="H-InvDB:HIT000100007"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH00696X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..543
                     /codon_start=1
                     /product="ADP-ribosylation factor 5"
                     /protein_id="AAP35750.1"
                     /translation="MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVT
                     TIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQE
                     SADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCA
                     TQGTGLYDGLDWLSHELSKR"
BASE COUNT          128 a          142 c          162 g          111 t
ORIGIN      
        1 atgggcctca ccgtgtccgc gctcttttcg cggatcttcg ggaagaagca gatgcggatt
       61 ctcatggttg gcttggatgc ggctggcaag accacaatcc tgtacaaact gaagttgggg
      121 gagattgtca ccaccatccc aaccataggc ttcaatgtag aaacagtgga atataagaac
      181 atctgtttca cagtctggga cgtgggaggc caggacaaga ttcggcctct gtggcggcac
      241 tacttccaga acactcaggg cctcatcttt gtggtggaca gtaatgaccg ggagcgggtc
      301 caagaatctg ctgatgaact ccagaagatg ctgcaggagg acgagctgcg ggatgcagtg
      361 ctgctggtat ttgccaacaa gcaggacatg cccaacgcca tgcccgtgag cgagctgact
      421 gacaagctgg ggctacagca cttacgcagc cgcacgtggt atgtccaggc cacctgtgcc
      481 acccaaggca caggtctgta cgatggtctg gactggctgt cccacgagct gtcaaagcgc
      541 tag
//