LOCUS       BT007084                 510 bp    mRNA    linear   HUM 13-MAY-2003
DEFINITION  Homo sapiens ethanolamine kinase mRNA, complete cds.
ACCESSION   BT007084
VERSION     BT007084.1
KEYWORDS    FLI_CDNA.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Cloning of human full-length CDSs in BD Creator(TM) System Donor
            vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 510)
  AUTHORS   Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
            Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
            Phelan,M. and Farmer,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
            Circle, Palo Alto, CA 94303, USA
COMMENT     This CDS clone is a part of a collection of human full length
            expression clones generated by BD Biosciences Clontech and the
            Harvard Institute of Proteomics. Each CDS has been cloned in two
            forms: with and without stop-codon (to allow fusion with C-terminal
            tag). The CDS has been  directionally cloned using BD In-Fusion(TM)
            cloning system between the SalI  and HindIII sites of the pDNR-DUAL
            vector. Additional sequences in the clone:  'ACC' after SalI site
            and before 'ATG' to provide Kozak consensus sequence; 'GG' after
            last codon and before HindIII site to maintain reading frame.
            Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES             Location/Qualifiers
     source          1..510
                     /db_xref="H-InvDB:HIT000100004"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="GH00691X1.0"
                     /clone_lib="BD Creator(TM) CDS Library derived from MGC
                     collection"
                     /lab_host="DH5alpha T1 resistant"
                     /note="Vector: pDNR-Dual"
     CDS             1..510
                     /codon_start=1
                     /product="ethanolamine kinase"
                     /protein_id="AAP35747.1"
                     /translation="MANYIHVPPGSPEVPKLNVTVQDQEEHRCREGALSLLQHLRPHW
                     DPQEVTLQLFTDGITNKLIGCYVGNTMEDVVLVRIYGNKTELLVDRDEEVKSFRVLQA
                     HGCAPQLYCTFNNGLCYEFIQGEALDPKHVCNPAIFSLSSLTLCKGKTTRCFGLTGCR
                     GSRLLLSFF"
BASE COUNT          133 a          121 c          130 g          126 t
ORIGIN      
        1 atggccaatt acatccacgt ccctcccggc tccccggagg tgcccaagct gaacgtcacc
       61 gttcaggatc aggaggagca tcgctgccgg gagggggccc tgagcctcct gcaacacctg
      121 cggcctcact gggaccccca ggaggtgacc ctgcagctct tcacagatgg aatcacaaat
      181 aaacttattg gctgttacgt gggaaacacc atggaggatg tagtcctggt gagaatttat
      241 ggcaataaga ctgagttatt agtcgatcga gatgaggaag taaagagttt tcgagtgttg
      301 caggctcatg ggtgtgcacc acaactctac tgtaccttca ataatggact atgctatgaa
      361 tttatacaag gagaagcact ggatccaaag catgtctgca acccagccat tttcagttta
      421 tcatcgttga ctctttgcaa aggaaaaact acaagatgtt ttggattaac cggctgcaga
      481 gggtcaaggc ttctgcttag ttttttctag
//