LOCUS BT007026 3039 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens ubiquitin-activating enzyme E1-like mRNA, complete
cds.
ACCESSION BT007026
VERSION BT007026.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3039)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3039)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..3039
/db_xref="H-InvDB:HIT000099946"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00330X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..3039
/codon_start=1
/product="ubiquitin-activating enzyme E1-like"
/protein_id="AAP35672.1"
/translation="MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLG
AEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAV
QVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFC
DFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMV
ELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHV
VAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEE
EPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDAL
DCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKV
FALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQ
VIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSG
TWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFR
LSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFH
YGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHG
LPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWS
VGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATT
TAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSW
DRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVT
ELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL"
BASE COUNT 618 a 880 c 896 g 645 t
ORIGIN
1 atggatgccc tggacgcttc gaagctactg gatgaggagc tgtattcaag acagctgtat
61 gtgctgggct cacctgccat gcagaggatt cagggagcca gggtcctggt gtcaggcctg
121 cagggcctgg gggccgaggt ggccaagaac ttggttctga tgggtgtggg cagcctcact
181 ctgcatgatc cccaccccac ctgctggtcc gacctggctg cccagtttct cctctcagag
241 caggacttgg aaaggagcag agccgaggcc tctcaagagc tcttggctca gctcaacaga
301 gctgtccagg tcgtcgtgca cacgggtgac atcactgagg acctgctgtt ggacttccag
361 gtggtggtgc tgactgctgc aaagctggag gagcagctga aggtgggcac cttgtgtcat
421 aagcatggag tttgctttct ggcggctgac acccggggcc tcgtggggca gttgttctgt
481 gactttggtg aggacttcac tgtgcaggac cccacagagg cagaacccct gacagctgcc
541 atccagcaca tctcccaggg ctcccctggc attctcactc tgaggaaagg ggccaatacc
601 cactacttcc gtgatggaga cttggtgact ttctcgggaa ttgagggaat ggttgagctc
661 aacgactgtg atccccggtc tatccacgtg cgggaggatg ggtccctgga gattggagac
721 acaacaactt tctctcggta cttgcgtggt ggggctatca ctgaagtcaa gagacccaag
781 actgtgagac ataagtccct ggacacagcc ctgctccagc cccatgtggt ggcccagagc
841 tcccaggaag ttcaccatgc ccactgcctg catcaggcct tctgtgcact gcacaagttc
901 cagcacctcc atggccggcc accccagccc tgggatcctg ttgatgcaga gactgtggtg
961 ggcctggccc gggacctgga accactgaag cggacagagg aagagccact ggaagagcca
1021 ctggatgagg ccctagtgcg gacagtcgcc ctaagcagtg caggtgtctt gagccctatg
1081 gtggccatgc tgggtgcagt agctgcccag gaagtgctga aggcaatctc caggaagttc
1141 atgcctctgg accagtggct ttactttgat gccctcgatt gtcttccgga agatggggag
1201 ctccttccca gtcctgagga ctgtgccctg agaggcagcc gctatgatgg gcaaattgca
1261 gtgtttgggg ctggttttca ggagaaactg agacgccagc actacctcct ggtgggcgct
1321 ggtgccattg gttgtgagct gctcaaagtc tttgccctag tgggactggg ggccgggaac
1381 agcgggggct tgactgttgt tgacatggac cacatagagc gctccaatct cagccgtcag
1441 ttcctcttca ggtcccagga cgttggtaga cccaaggcag aggtggctgc agcagctgcc
1501 cggggcctga acccagactt acaggtgatc ccgctcacct acccactgga tcccaccaca
1561 gagcacatct atggggataa ctttttctcc cgtgtggatg gtgtggctgc tgccctggac
1621 agtttccagg cccggcgcta tgtggctgct cgttgcaccc actatctgaa gccactgctg
1681 gaggcaggca catcgggcac ctggggcagt gctacagtat tcatgccaca tgtgactgag
1741 gcctacagag cccctgcctc agctgcagct tctgaggatg ccccctaccc tgtctgtacc
1801 gtgcggtact tccctagcac agccgagcac accctgcagt gggcccggca tgagtttgaa
1861 gaactcttcc gactgtctgc agagaccatc aaccaccacc aacaggcaca cacttccctg
1921 gcagacatgg atgagccaca gacactcacc ttactgaagc cagtgcttgg ggtcctgaga
1981 gtgcgtccac agaactggca agactgtgtg gcgtgggctc ttggccactg gaaactctgc
2041 tttcattatg gcatcaaaca gctgctgagg cacttcccac ctaataaagt gcttgaggat
2101 ggaactccct tctggtcagg tcccaaacag tgtccccagc ccttggagtt tgacaccaac
2161 caagacacac acctcctcta cgtactggca gctgccaacc tgtatgccca gatgcatggg
2221 ctgcctggct cacaggactg gactgcactc agggagctgc tgaagctgct gccacagcct
2281 gacccccaac agatggcccc catctttgct agtaatctag agctggcttc ggcttctgct
2341 gagtttggcc ctgagcagca gaaggaactg aacaaagccc tggaagtctg gagtgtgggc
2401 cctcccctga agcctctgat gtttgagaag gatgatgaca gcaacttcca tgtggacttt
2461 gtggtagcgg cagctagcct gagatgtcag aactacggga ttccaccggt caaccgtgcc
2521 cagagcaagc gaattgtggg ccagattatc ccagccattg ccaccactac agcagctgtg
2581 gcaggcctgt tgggcctgga gctgtataag gtggtgagtg ggccacggcc tcgtagtgcc
2641 tttcgccaca gctacctaca tctggctgaa aactacctca tccgctatat gccttttgcc
2701 ccagccatcc agacgttcca tcacctgaag tggacctctt gggaccgtct gaaggtacca
2761 gctgggcagc ctgagaggac cctggagtcg ctgctggctc atcttcagga gcagcacggg
2821 ttgagggtga ggatcctgct gcacggctca gccctgctct atgcggccgg atggtcacct
2881 gaaaagcagg cccagcacct gcccctcagg gtgacagaac tggttcagca gctgacaggc
2941 caggcacctg ctcctgggca gcgggtgttg gtgctagagc tgagctgtga gggtgacgac
3001 gaggacactg ccttcccacc tctgcactat gagctgtag
//