LOCUS BT006872 1977 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens endoglin (Osler-Rendu-Weber syndrome 1) mRNA, complete
cds.
ACCESSION BT006872
VERSION BT006872.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1977)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1977)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1977
/db_xref="H-InvDB:HIT000099792"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00467X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1977
/codon_start=1
/product="endoglin (Osler-Rendu-Weber syndrome 1)"
/protein_id="AAP35518.1"
/translation="MDRGTLPLAVALLLASCSLSPTSLAETVHCDLQPVGPERDEVTY
TTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVN
SSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAA
ELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEA
HILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGE
YSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRL
QTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDP
SCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSF
QLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQ
GRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHR
TVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSKRE
PVVAVAAPASSESSSTNHSIGSTQSTPCSTSSMA"
BASE COUNT 390 a 677 c 536 g 374 t
ORIGIN
1 atggaccgcg gcacgctccc tctggctgtt gccctgctgc tggccagctg cagcctcagc
61 cccacaagtc ttgcagaaac agtccattgt gaccttcagc ctgtgggccc cgagagggac
121 gaggtgacat ataccactag ccaggtctcg aagggctgcg tggctcaggc ccccaatgcc
181 atccttgaag tccatgtcct cttcctggag ttcccaacgg gcccgtcaca gctggagctg
241 actctccagg catccaagca aaatggcacc tggccccgag aggtgcttct ggtcctcagt
301 gtaaacagca gtgtcttcct gcatctccag gccctgggaa tcccactgca cttggcctac
361 aattccagcc tggtcacctt ccaagagccc ccgggggtca acaccacaga gctgccatcc
421 ttccccaaga cccagatcct tgagtgggca gctgagaggg gccccatcac ctctgctgct
481 gagctgaatg acccccagag catcctcctc cgactgggcc aagcccaggg gtcactgtcc
541 ttctgcatgc tggaagccag ccaggacatg ggccgcacgc tcgagtggcg gccgcgtact
601 ccagccttgg tccggggctg ccacttggaa ggcgtggccg gccacaagga ggcgcacatc
661 ctgagggtcc tgccgggcca ctcggccggg ccccggacgg tgacggtgaa ggtggaactg
721 agctgcgcac ccggggatct cgatgccgtc ctcatcctgc agggtccccc ctacgtgtcc
781 tggctcatcg acgccaacca caacatgcag atctggacca ctggagaata ctccttcaag
841 atctttccag agaaaaacat tcgtggcttc aagctcccag acacacctca aggcctcctg
901 ggggaggccc ggatgctcaa tgccagcatt gtggcatcct tcgtggagct accgctggcc
961 agcattgtct cacttcatgc ctccagctgc ggtggtaggc tgcagacctc acccgcaccg
1021 atccagacca ctcctcccaa ggacacttgt agcccggagc tgctcatgtc cttgatccag
1081 acaaagtgtg ccgacgacgc catgaccctg gtactaaaga aagagcttgt tgcgcatttg
1141 aagtgcacca tcacgggcct gaccttctgg gaccccagct gtgaggcaga ggacaggggt
1201 gacaagtttg tcttgcgcag tgcttactcc agctgtggca tgcaggtgtc agcaagtatg
1261 atcagcaatg aggcggtggt caatatcctg tcgagctcat caccacagcg gaaaaaggtg
1321 cactgcctca acatggacag cctctctttc cagctgggcc tctacctcag cccacacttc
1381 ctccaggcct ccaacaccat cgagccgggg cagcagagct ttgtgcaggt cagagtgtcc
1441 ccatccgtct ccgagttcct gctccagtta gacagctgcc acctggactt ggggcctgag
1501 ggaggcaccg tggaactcat ccagggccgg gcggccaagg gcaactgtgt gagcctgctg
1561 tccccaagcc ccgagggtga cccgcgcttc agcttcctcc tccacttcta cacagtaccc
1621 atacccaaaa ccggcaccct cagctgcacg gtagccctgc gtcccaagac cgggtctcaa
1681 gaccaggaag tccataggac tgtcttcatg cgcttgaaca tcatcagccc tgacctgtct
1741 ggttgcacaa gcaaaggcct cgtcctgccc gccgtgctgg gcatcacctt tggtgccttc
1801 ctcatcgggg ccctgctcac tgctgcactc tggtacatct actcgcacac gcgttccccc
1861 agcaagcggg agcccgtggt ggcggtggct gccccggcct cctcggagag cagcagcacc
1921 aaccacagca tcgggagcac ccagagcacc ccctgctcca ccagcagcat ggcatag
//