LOCUS BT006849 1575 bp mRNA linear HUM 13-MAY-2003 DEFINITION Homo sapiens prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) mRNA, complete cds. ACCESSION BT006849 VERSION BT006849.1 KEYWORDS FLI_CDNA. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1575) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor vector JOURNAL Unpublished REFERENCE 2 (bases 1 to 1575) AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S., Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y., Phelan,M. and Farmer,A. TITLE Direct Submission JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow Circle, Palo Alto, CA 94303, USA COMMENT This CDS clone is a part of a collection of human full length expression clones generated by BD Biosciences Clontech and the Harvard Institute of Proteomics. Each CDS has been cloned in two forms: with and without stop-codon (to allow fusion with C-terminal tag). The CDS has been directionally cloned using BD In-Fusion(TM) cloning system between the SalI and HindIII sites of the pDNR-DUAL vector. Additional sequences in the clone: 'ACC' after SalI site and before 'ATG' to provide Kozak consensus sequence; 'GG' after last codon and before HindIII site to maintain reading frame. Clone distribution: http://bioinfo.clontech.com/orfclones. FEATURES Location/Qualifiers source 1..1575 /db_xref="H-InvDB:HIT000099769" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="GH00495X1.0" /clone_lib="BD Creator(TM) CDS Library derived from MGC collection" /lab_host="DH5alpha T1 resistant" /note="Vector: pDNR-Dual" CDS 1..1575 /codon_start=1 /product="prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy)" /protein_id="AAP35495.1" /translation="MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGA VKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNM SASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIP ELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTF VQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKE MPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKT EKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTR LPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQ CDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTET AAQCNAVEHCKRHVWN" BASE COUNT 374 a 419 c 451 g 331 t ORIGIN 1 atgtacgccc tcttcctcct ggccagcctc ctgggcgcgg ctctagccgg cccggtcctt 61 ggactgaaag aatgcaccag gggctcggca gtgtggtgcc agaatgtgaa gacggcgtcc 121 gactgcgggg cagtgaagca ctgcctgcag accgtttgga acaagccaac agtgaaatcc 181 cttccctgcg acatatgcaa agacgttgtc accgcagctg gtgatatgct gaaggacaat 241 gccactgagg aggagatcct tgtttacttg gagaagacct gtgactggct tccgaaaccg 301 aacatgtctg cttcatgcaa ggagatagtg gactcctacc tccctgtcat cctggacatc 361 attaaaggag aaatgagccg tcctggggag gtgtgctctg ctctcaacct ctgcgagtct 421 ctccagaagc acctagcaga gctgaatcac cagaagcagc tggagtccaa taagatccca 481 gagctggaca tgactgaggt ggtggccccc ttcatggcca acatccctct cctcctctac 541 cctcaggacg gcccccgcag caagccccag ccaaaggata atggggacgt ttgccaggac 601 tgcattcaga tggtgactga catccagact gctgtacgga ccaactccac ctttgtccag 661 gccttggtgg aacatgtcaa ggaggagtgt gaccgcctgg gccctggcat ggccgacata 721 tgcaagaact atatcagcca gtattctgaa attgctatcc agatgatgat gcacatgcaa 781 cccaaggaga tctgtgcgct ggttgggttc tgtgatgagg tgaaagagat gcccatgcag 841 actctggtcc ccgccaaagt ggcctccaag aatgtcatcc ctgccctgga actggtggag 901 cccattaaga agcacgaggt cccagcaaag tctgatgttt actgtgaggt gtgtgaattc 961 ctggtgaagg aggtgaccaa gctgattgac aacaacaaga ctgagaaaga aatactcgac 1021 gcttttgaca aaatgtgctc gaagctgccg aagtccctgt cggaagagtg ccaggaggtg 1081 gtggacacgt acggcagctc catcctgtcc atcctgctgg aggaggtcag ccctgagctg 1141 gtgtgcagca tgctgcacct ctgctctggc acgcggctgc ctgcactgac cgttcacgtg 1201 actcagccaa aggacggtgg cttctgcgaa gtgtgcaaga agctggtggg ttatttggat 1261 cgcaacctgg agaaaaacag caccaagcag gagatcctgg ctgctcttga gaaaggctgc 1321 agcttcctgc cagaccctta ccagaagcag tgtgatcagt ttgtggcaga gtacgagccc 1381 gtgctgatcg agatcctggt ggaggtgatg gatccttcct tcgtgtgctt gaaaattgga 1441 gcctgcccct cggcccataa gcccttgttg ggaactgaga agtgtatatg gggcccaagc 1501 tactggtgcc agaacacaga gacagcagcc cagtgcaatg ctgtcgagca ttgcaaacgc 1561 catgtgtgga actag //