LOCUS BT006697 1299 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens S-adenosylhomocysteine hydrolase mRNA, complete cds.
ACCESSION BT006697
VERSION BT006697.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1299)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1299)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..1299
/db_xref="H-InvDB:HIT000099617"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00082X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..1299
/codon_start=1
/product="S-adenosylhomocysteine hydrolase"
/protein_id="AAP35343.1"
/translation="MSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKP
LKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAW
KGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTG
VHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVV
AGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTT
GCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGR
RIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDE
AVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY"
BASE COUNT 288 a 369 c 391 g 251 t
ORIGIN
1 atgtctgaca aactgcccta caaagtcgcc gacatcggcc tggctgcctg gggacgcaag
61 gccctggaca ttgctgagaa cgagatgccg ggcctgatgc gtatgcggga gcggtactcg
121 gcctccaagc cactgaaggg cgcccgcatc gctggctgcc tgcacatgac cgtggagacg
181 gccgtcctca ttgagaccct cgtcaccctg ggtgctgagg tgcagtggtc cagctgcaac
241 atcttctcca cccaggacca tgcggcggct gccattgcca aggctggcat tccggtgtat
301 gcctggaagg gcgaaacgga cgaggagtac ctgtggtgca ttgagcagac cctgtacttc
361 aaggacgggc ccctcaacat gattctggac gacgggggcg acctcaccaa cctcatccac
421 accaagtacc cgcagcttct gccaggcatc cgaggcatct ctgaggagac cacgactggg
481 gtccacaacc tctacaagat gatggccaat gggatcctca aggtgcctgc catcaatgtc
541 aatgactccg tcaccaagag caagtttgac aacctctatg gctgccggga gtccctcata
601 gatggcatca agcgggccac agatgtgatg attgccggca aggtagcggt ggtagcaggc
661 tatggtgatg tgggcaaggg ctgtgcccag gccctgcggg gtttcggagc ccgcgtcatc
721 atcaccgaga ttgaccccat caacgcactg caggctgcca tggagggcta tgaggtgacc
781 accatggatg aggcctgtca ggagggcaac atctttgtca ccaccacagg ctgtattgac
841 atcatccttg gccggcactt tgagcagatg aaggatgatg ccattgtgtg taacattgga
901 cactttgacg tggagatcga tgtcaagtgg ctcaacgaga acgccgtgga gaaggtgaac
961 atcaagccgc aggtggaccg gtatcggttg aagaatgggc gccgcatcat cctgctggcc
1021 gagggtcggc tggtcaacct gggttgtgcc atgggccacc ccagcttcgt gatgagtaac
1081 tccttcacca accaggtgat ggcgcagatc gagctgtgga cccatccaga caagtacccc
1141 gttggggttc atttcctgcc caagaagctg gatgaggcag tggctgaagc ccacctgggc
1201 aagctgaatg tgaagttgac caagctaact gagaagcaag cccagtacct gggcatgtcc
1261 tgtgatggcc ccttcaagcc ggatcactac cgctactag
//