LOCUS BT006663 2058 bp mRNA linear HUM 13-MAY-2003
DEFINITION Homo sapiens midline 2 mRNA, complete cds.
ACCESSION BT006663
VERSION BT006663.1
KEYWORDS FLI_CDNA.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2058)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Cloning of human full-length CDSs in BD Creator(TM) System Donor
vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2058)
AUTHORS Kalnine,N., Chen,X., Rolfs,A., Halleck,A., Hines,L., Eisenstein,S.,
Koundinya,M., Raphael,J., Moreira,D., Kelley,T., LaBaer,J., Lin,Y.,
Phelan,M. and Farmer,A.
TITLE Direct Submission
JOURNAL Submitted (13-MAY-2003) BD Biosciences Clontech, 1020 East Meadow
Circle, Palo Alto, CA 94303, USA
COMMENT This CDS clone is a part of a collection of human full length
expression clones generated by BD Biosciences Clontech and the
Harvard Institute of Proteomics. Each CDS has been cloned in two
forms: with and without stop-codon (to allow fusion with C-terminal
tag). The CDS has been directionally cloned using BD In-Fusion(TM)
cloning system between the SalI and HindIII sites of the pDNR-DUAL
vector. Additional sequences in the clone: 'ACC' after SalI site
and before 'ATG' to provide Kozak consensus sequence; 'GG' after
last codon and before HindIII site to maintain reading frame.
Clone distribution: http://bioinfo.clontech.com/orfclones.
FEATURES Location/Qualifiers
source 1..2058
/db_xref="H-InvDB:HIT000099583"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="GH00646X1.0"
/clone_lib="BD Creator(TM) CDS Library derived from MGC
collection"
/lab_host="DH5alpha T1 resistant"
/note="Vector: pDNR-Dual"
CDS 1..2058
/codon_start=1
/product="midline 2"
/protein_id="AAP35309.1"
/translation="METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSS
GESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRR
ERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHR
LVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEK
LKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQR
KQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKN
IAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELC
TASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTV
HGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEK
DESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNE
WIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSL
HLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMK
TCH"
BASE COUNT 583 a 516 c 461 g 498 t
ORIGIN
1 atggaaacac tggagtctga attgacctgt ccaatctgcc tagagttgtt tgaagacccc
61 cttctgctcc cttgtgctca cagcctctgc ttcagctgtg cccatcgcat tttggtatca
121 agctgcagct ctggtgaatc cattgaaccc attactgctt tccagtgtcc tacctgcagg
181 tatgttatct cgctgaacca ccggggcctg gatggcctca agaggaatgt gactctgcag
241 aacattattg atcgcttcca gaaggcttca gtcagtgggc ccaattcccc tagtgagagc
301 cgccgggaaa ggacttacag gcccaccact gccatgtcta gcgagcgaat tgcttgccaa
361 ttctgtgagc aggacccgcc aagggatgca gtaaaaacat gcatcacctg tgaggtctcc
421 tactgtgacc gttgcctgcg ggccacgcac cccaacaaga aacctttcac cagccaccgc
481 ctggtggaac cagtgccaga cacacatctt cgagggatca cctgcctgga ccatgagaat
541 gagaaagtga acatgtactg tgtatctgat gaccaattga tctgtgcctt atgcaaactg
601 gtgggtcgtc accgagacca tcaggtcgca tccctgaatg atcgatttga gaaactcaag
661 caaactctgg agatgaacct caccaacctg gttaagcgca acagcgaact agaaaatcaa
721 atggccaaac taatacagat ctgccagcag gttgaggtga atactgctat gcatgaggca
781 aaacttatgg aagaatgtga cgagttggta gagatcatcc agcagaggaa gcaaatgatc
841 gctgtcaaaa tcaaagagac aaaggttatg aaactgagaa agttggcaca gcaggttgct
901 aattgccgcc agtgtcttga acggtcaaca gtcctcatca accaagctga gcatatcctg
961 aaagaaaatg accaggcacg gtttctacag tctgcaaaaa atattgctga gagggtcgct
1021 atggcaactg catcttctca agttctgatt ccagacatca attttaatga tgcctttgaa
1081 aactttgctt tagatttttc cagagaaaag aaactgctag aggggttaga ttatttaaca
1141 gccccaaacc caccatctat ccgagaagaa ctctgtactg cctcccatga caccattaca
1201 gtccactgga tctcggatga tgagttcagc atcagctcct atgagcttca gtacaccata
1261 ttcactggcc aggctaactt catcagcctg tataattcag tagacagctg gatgattgtt
1321 cccaacatta aacagaacca ttacacagtg catggactcc agagcgggac tcgctacatc
1381 ttcatcgtta aagccataaa ccaagccggc agccggaaca gtgaacctac ccgactaaaa
1441 acaaacagcc aaccctttaa attggatccc aaaatgactc acaagaagtt gaagatctcc
1501 aatgatggat tgcagatgga gaaggatgaa agctctctaa agaagagcca caccccagag
1561 aggtttagtg gcacagggtg ctatggggca gcaggaaata tattcattga cagtggctgc
1621 cactattggg aggtggtcat gggttcctca acatggtatg caattggcat tgcctacaaa
1681 tcagctccaa agaatgaatg gattggcaag aatgcctcct catgggtctt ctctcgctgc
1741 aatagtaact tcgtggtgag acacaacaac aaggaaatgc tggtggatgt gcccccacac
1801 ctgaagcgtc tgggtgtcct cctggattat gacaacaata tgctgtcttt ctatgaccca
1861 gctaactctc tccatcttca tacttttgat gtgaccttca ttcttccagt ttgtccaaca
1921 tttacaatct ggaacaaatc cctaatgatc ctgtctggct tgcctgcccc agattttatt
1981 gattaccctg agcggcagga atgcaactgc aggcctcaag aatcccctta tgtttctggg
2041 atgaaaacct gtcattag
//