LOCUS BQ025444 706 bp mRNA linear EST 07-JAN-2011
DEFINITION UI-1-BB1p-aua-c-04-0-UI.s1 NCI_CGAP_Pl6 Homo sapiens cDNA clone
UI-1-BB1p-aua-c-04-0-UI 3', mRNA sequence.
ACCESSION BQ025444
VERSION BQ025444.1
DBLINK BioSample: SAMN00170300
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 706)
CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished
COMMENT Contact: Robert Strausberg, Ph.D.
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Dr. Steven Brown
cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
Clone Distribution: Clone distribution information can be obtained
from Dr. M. Bento Soares, bento-soares@uiowa.edu
The following repetitive elements were found in this cDNA
sequence: 1-38, >AT_rich#Low_complexity (matched compliment)
570-655, >ALU 656-688, >(GAAAA)n#Simple_repeat
Seq primer: M13 FORWARD
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..706
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="UI-1-BB1p-aua-c-04-0-UI"
/tissue_type="Placenta"
/clone_lib="SAMN00170300 NCI_CGAP_Pl6"
/dev_stage="Full Term"
/lab_host="DH10B (Life Technologies)"
/note="Organ: Placenta; Vector: pT7T3-Pac (Pharmacia) with
a modified polylinker; Site_1: EcoR I; Site_2: Not I;
NCI_CGAP_Pl6 is a subtracted cDNA library constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tags for this library are GA, AGGAA. For
additional information, contact: Bento Soares,
bento-soares@uiowa.edu;
TAG_LIB=UI-1-BB1p;
TAG_SEQ=AGGAA;
TAG_TISSUE=placenta human full term"
BASE COUNT 225 a 132 c 147 g 200 t
ORIGIN
1 tttttttttt ttttttttag tattacattt tatttttact gcttcttcca gtattgcaca
61 atcccataat tattgctatt cagctaaaaa catgcctcga ggcattgcag aagatgagaa
121 taataatttt aaaaactgga gtcagcaaca cgaactcttg aaagcactag tataaaagca
181 ttcaagaaat aacttcctct ggcttccttg gacttgttta agagattact aatggcaaaa
241 tcaagtggaa attttaatgg atcagtaggt ctttatgaac tataagttcc ccagatgaaa
301 atcaaaatgc tgctcgtgat gtatgcctta gaacaggatt gctgacctaa ggtctgggaa
361 cctgctaagg ctgtgggctt atgcactgag tatttgagag catttttctg aaaaggggag
421 cacatagctc tcaactactc tccaaggggg tcccccaaat gttaaattta gagtttgctt
481 agttgacagc agagtttccc gatcgggttc ccttttgttg ttctagtgaa caggtaaaaa
541 ctcagtgaaa ggcagactgg gaatgttctc ctgggaggtg gaggttgcag tgagccgaga
601 tcgcaccact gcacacccgc ctgggcaaca gaccgagact ccatctcana anaaaaaaaa
661 aaagaaaaga aaagaaaaag aaaataaata ttgagcaccc taggca
//