LOCUS BM713594 578 bp mRNA linear EST 28-JAN-2011
DEFINITION UI-E-EJ0-aho-h-24-0-UI.r1 UI-E-EJ0 Homo sapiens cDNA clone
UI-E-EJ0-aho-h-24-0-UI 5', mRNA sequence.
ACCESSION BM713594
VERSION BM713594.1
DBLINK BioSample: SAMN00170185
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 578)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
Tissue Procurement: Dr. Gregg Hageman
cDNA Library preparation: Dr. M. Bento Soares, Univeristy of Iowa
cDNA Library Arrayed by: Dr. M. Bento Soares, Univeristy of Iowa
DNA Sequencing by: Dr. M. Bento Soares, Univeristy of Iowa
Clone Distribution: Researchers may obtain clones from Research
Genetics (www.resgen.com).
Seq primer: M13 Reverse.
FEATURES Location/Qualifiers
source 1..578
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="UI-E-EJ0-aho-h-24-0-UI"
/tissue_type="fetal eyes, lens, eye anterior segment,
optic nerve, retina, Retina Foveal and Macular, RPE and
Choroid"
/clone_lib="SAMN00170185 UI-E-EJ0"
/dev_stage="fetal and adult"
/lab_host="DH10B (Life Technologies) (T1 phage resistant)"
/note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
modified polylinker; Site_1: EcoR I; Site_2: Not I;
UI-E-EJ0 is a subtracted cDNA library constructed
according to Bonaldo, Lennon and Soares, Genome Research,
6:791-806, 1996. First strand cDNA synthesis was primed
with an oligo-dT primer containing a Not I site. Double
stranded cDNA was ligated to an EcoR I adaptor, digested
with Not I, and cloned directionally into pT7T3-Pac
vector. The oligonucleotide used to prime the synthesis of
first-strand cDNA contains a library tag sequence that is
located between the Not I site and the (dT)18 tail. The
sequence tags for this library are: fetal eyes,
AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
library was created for the program, Gene Discovery in the
Visual System, supported by National Eye Institute (NEI)."
BASE COUNT 131 a 142 c 184 g 121 t
ORIGIN
1 gcggaaatgg ctgggagggg gatggggatg ttgaatttgg aaattggagg ggacgctggt
61 ggacggggct ctctgtgtgg cttgtaagat gctgaagact cagattttag agaccaagtg
121 cgtttcagaa gagggctgag gattgggtgc aaggagttgg tgttgatgga ggagcaggac
181 gccagagtcc cagccctgga accgttcaga gtggagcagg caccacctgt aatctactat
241 gtccctgact tcatctccaa agaagaggag gagtatttgc ttcgacaggt ttttaatgcc
301 ccaaagccaa agtggaccca gctctctggg agaaagttac agaactgggg tgggcttcct
361 catccccgag ggatggttcc tgagcggctg cccccatggc tccagcgcta cgtggacaaa
421 gtgtcaaacc tcagcctctt tggaggcctc ccagctaacc atgtcctcgt gaaccagtat
481 ctgcctgggg agggcatcat gcaccatcag cctgggctcc cacaccgtgc tggacttcta
541 cgagccgcgg cggccagagg acgatgaccc tacagaac
//